STK3 (NM_006281) Human Untagged Clone

CAT#: SC323560

STK3 (untagged)-Kinase deficient mutant (K56M) of Human serine/threonine kinase 3 (STE20 homolog, yeast) (STK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK3
• Synonyms: KRS1; MST2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006281, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTG
  AAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAA
  GGAATCCGGTCAAGTTGTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAA
  ATTTCCATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATACAG
  ACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGAC
  ATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGACTAGAATATTTGCACTTTATG
  AGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCAAAATTGGCAG
  ATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTG
  GATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACT
  TCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATGATTC
  CCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATTTTGTTAAAAA
  GTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAAT
  GCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGAGG
  AACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGT
  GAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATG
  ATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAG
  AAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTT
  TGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATG
  TCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTGAAAAATCTAA
  GTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCG
  TCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAAC
  TTTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006281 unedited
  CCCGCCCGTATCAGCAACTGGGCGGTAGGCGCTGTACGGTTGGGAGGCTCTATATAAGCAGAGCTCGTTT
  AGTGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGGCCGCCGTC
  CACCAGGCCCCTCTCTCTGTCCCCGGCCGCCATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAG
  CTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGCTTTTGATGTATTAGAGAAGCTTGGAGAAGGGT
  CTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTTGTCGCAATTATGCAAGTACCTGT
  TGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATGCAGCATGGTGACAGCCCATATGTTGTA
  AAGTACTATGGCAGTTTATTTTAAGAATACCAGACTCTGAATGGTTATGGAAGTACTGGGCCCTGCTTTG
  TTTAGACTTATTAGATACGAAACAGACATTATAGAAAATGAATTTGCACCTTCTTAATCCACATGGAAGG
  CTAGATTTTTGACTTTTGAAAAATCCCGGATTTAACCTGAAATTCTCTAATACAAAGGACTGCAATGGCA
  ATTTGATGCCGGTCTTACGATACAGGCAACGCAACTGTATAGACCCATTGGAGGCCGGGGTACGAATGCT
  AACGTGGGCAATTGCCTTGGTATTTTGAAAGCGAGGACCTTGCGTATCCTAGGGCTTTATCAATCACACT
  ACACCTGCGATCCAATCACGTGGATCCGAACTGCACTAGCTGAAGACGTAA
  
  Kinase domain sequence

  >SC323560 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCGCCGTCCACCAGGTCCC
  TCTCTCTGTCCCCGGCCGCCATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGA
  CAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_006281
• Insert Size: 2730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006281.1, NP_006272.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1476 bp
• Locus ID: 6788
• Cytogenetics: 8q22.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) encodes isoform 1.