PEG3 (NM_001146185) Human Untagged Clone

CAT#: SC327712

PEG3 (untagged)-Human paternally expressed 3 (PEG3) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEG3
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146185, the custom clone sequence may differ by one or more nucleotides
  ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGG
  AACAGAAGAGAGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGACCGGGACTGGGAC
  CGGAGGGGCAGAAGCAGAGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCA
  AGAAGCAGGATGCCTCCGCGGGATCTTTCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAA
  ATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATGAGTCCCGATCTCAGGATGCTGAA
  TCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAACACAATCCAGGAC
  AACATGGAAAACTACAGGAAGCTGCTCTCCCTCGTGCAGCTTGCTGAAGACGATGGCCAC
  TCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGCACCAGT
  CGAGGTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATTTGTGAA
  GATGAATCTTCCCACGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCC
  AAATCGGGAAGAGCAAGGGAGTCAAGCGACCGGTCACAGAGATTCCCCAGAATGTCAGAT
  GATAACTGGAAGGACATTTCATTGAACAAGAGGGAGTCAGTGATCCAGCAGCGGGTTTAT
  GAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCTTGTTTCCAGAAAGAGA
  GTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATTCACGAT
  CAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAAGCCATG
  AGCGTGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCAATTGAT
  TTTGGGGCAATGCCATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAA
  TTTGTTGAGCACCAGATCATGCATACTAGAGAGAACCTCTATGAGTATGGTGAGTCCTTT
  ATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAAGTCAGGTTGGAGGGAAACGTTTTGAA
  TGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGCTGAACATCGGAAGATT
  CATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTCATGCCT
  AGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAGTGCAGG
  GTGTGTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATCCACTTT
  GGGGATGACAAAGATAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAA
  ACCTTTAGGCCCAGCCCAGCCCTTAATGAGTTTCAGAAAATGTATGGTAAAGAGAAAATG
  TACGAATGTAAGGTGTGTGGGGAGACTTTCCTTCATAGCTCATCCCTGAAAGAACATCAG
  AAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGTGTGTGAGGAAACCTTT
  ATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTCTGTGAC
  TTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAGAAAATT
  CATTCTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCATAGTGGG
  CCATTCACTGAATCTCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGAC
  GAAAAGGCGTTCACCATTAGCTCTAACCCCTATGAAAACCAGAAGATTCCCACTAAGGAA
  AATGTCTATGAGGCAAAATCATATGAGAGGTCTGTTATTCATAGCTTAGCCTCTGTGGAA
  GCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGTAATGGCAGAGTCTACC
  ATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACCTCTGAA
  GGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCAAGAAGT
  CACAATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTATATCTCA
  GACCTTAATGATAAGCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGT
  AAGAATCGCAACTATGAAGACTCTGTCATACAGAGTGTATTCCGTGCCAAACCTCAGAAA
  AGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGAAGGATGGCGAATTCTCTGTTCCCAGC
  TCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATACATTGAGCATAGGAGC
  AATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCTCGAGGG
  ATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTCACTGAG
  CACCAGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGGTCTGTC
  ATTCGCAGCTTGGCCCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAA
  GAGCAAGCGCGGAACAAATGTAAGGACTTCAGACAATTTTTTGCTACCAGCGAAGACCTC
  AACACAAACCAGAAAATCTATGACCAAGAGAAGTCTCATGGCGAGGAGTCTCAAGGCGAG
  AATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCAGGAGACAATTGAAGAC
  CCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGACAAAATC
  TATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGACCATCAG
  AAAGTCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTAATTCAC
  ACCCATTCCATCAGCGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCA
  AAGTGTGGGGAATCTTTTATTCATAGCTCATTCCTTTTCGAGCATCAGAGAATCCATGAA
  CAAGACCAGTTGTATTCCATGAAGGGGTGTGATGATGGTTTTATTGCCCTCTTGCCCATG
  AAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGCTGGGTCGGCCATTCGA
  TGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATGAGACTT
  CATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATTCCAGGC
  TTAGCCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGTGCAGTC
  TGTGGAGAATCTTTCGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAAT
  GAGCCCTATGAGTACGGGTCCTCCTATACTCACACCTCATTTCTTACTGAGCCCCTCAAA
  GGAGCTATACCATTCTATGAATGCAAGGATTGTGGTAAGTCCTTTATTCATAGCACAGTC
  CTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGATGAAGCAGCAGCAGCT
  GCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTTCTGAGG
  ATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCAGAAGTG
  GAGGCTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGGCCAGAT
  GGAGAGGCTGCAGAGCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCA
  AATGGGGATGCTGATGAGCCAGATGGTGCAGGTATTGAAGACCCAGAAGAAAGAGCTGAA
  GAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAGATGCCGACGAGCCTGACGGTGTGGGA
  ATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGAAGAACCATACTATGAC
  TGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTGAAAACT
  CATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGCTACATC
  GAACGTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTCAAATGT
  GACGTCTGTGGGCAGCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCAC
  ACTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001146185
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146185.1, NP_001139657.1
• RefSeq Size: 8375 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5178
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• Gene Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (3) is missing a 5' non-coding exon, and uses an alternate acceptor splice site at the first coding exon compared to variant 1. This results in the use of an in-frame downstream start codon, and an isoform (2) with a shorter N-terminus compared to isoform 1.