PEG3 (NM_001146185) Human Tagged ORF Clone

CAT#: RC229077

PEG3 (Myc-DDK-tagged)-Human paternally expressed 3 (PEG3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PEG3
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229077 representing NM_001146185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGGAACAGAAGAG
  AGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGACCGGGACTGGGACCGGAGGGGCAGAAGCAGAGA
  CATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAGAAGCAGGATGCCTCCGCGGGATCTTTCC
  CTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATGAGT
  CCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAACAC
  AATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGTGCAGCTTGCTGAAGACGATGGCCAC
  TCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGCACCAGTCGAGGTCTAA
  AAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATTTGTGAAGATGAATCTTCCCACGGAGT
  GATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCCAAATCGGGAAGAGCAAGGGAGTCAAGCGAC
  CGGTCACAGAGATTCCCCAGAATGTCAGATGATAACTGGAAGGACATTTCATTGAACAAGAGGGAGTCAG
  TGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCTTGTTTC
  CAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATTCACGAT
  CAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAAGCCATGAGCGTGAGCA
  GCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCAATTGATTTTGGGGCAATGCCATATGT
  ATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAATTTGTTGAGCACCAGATCATGCATACTAGA
  GAGAACCTCTATGAGTATGGTGAGTCCTTTATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAAGTCAGG
  TTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGCTGAACA
  TCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTCATGCCT
  AGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAGTGCAGGGTGTGTAAGG
  AAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATCCACTTTGGGGATGACAAAGATAATGA
  GCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAAACCTTTAGGCCCAGCCCAGCCCTTAATGAG
  TTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAATGTAAGGTGTGTGGGGAGACTTTCCTTCATAGCT
  CATCCCTGAAAGAACATCAGAAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGTGTGTGA
  GGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTCTGTGAC
  TTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAGAAAATTCATTCTCGAA
  AGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCATAGTGGGCCATTCACTGAATCTCAGAA
  GAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGACGAAAAGGCGTTCACCATTAGCTCTAACCCC
  TATGAAAACCAGAAGATTCCCACTAAGGAAAATGTCTATGAGGCAAAATCATATGAGAGGTCTGTTATTC
  ATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGTAATGGC
  AGAGTCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACCTCTGAA
  GGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCAAGAAGTCACAATGGAA
  ATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTATATCTCAGACCTTAATGATAAGCGACA
  GAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGTAAGAATCGCAACTATGAAGACTCTGTCATA
  CAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGAAGGATG
  GCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATACATTGA
  GCATAGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCTCGAGGG
  ATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTCACTGAGCACCAGAAGA
  TTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGGTCTGTCATTCGCAGCTTGGCCCCTAC
  TGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAAGAGCAAGCGCGGAACAAATGTAAGGACTTC
  AGACAATTTTTTGCTACCAGCGAAGACCTCAACACAAACCAGAAAATCTATGACCAAGAGAAGTCTCATG
  GCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCAGGAGAC
  AATTGAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGACAAAATC
  TATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGACCATCAGAAAGTCCACA
  GCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTAATTCACACCCATTCCATCAGCGAGTA
  TCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCAAAGTGTGGGGAATCTTTTATTCATAGCTCA
  TTCCTTTTCGAGCATCAGAGAATCCATGAACAAGACCAGTTGTATTCCATGAAGGGGTGTGATGATGGTT
  TTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGCTGGGTC
  GGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATGAGACTT
  CATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATTCCAGGCTTAGCCCTCA
  CTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGTGCAGTCTGTGGAGAATCTTTCGTCAA
  CCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAATGAGCCCTATGAGTACGGGTCCTCCTATACT
  CACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCTATACCATTCTATGAATGCAAGGATTGTGGTAAGT
  CCTTTATTCATAGCACAGTCCTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGATGAAGC
  AGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTTCTGAGG
  ATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCAGAAGTGGAGGCTGCTG
  AGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGGCCAGATGGAGAGGCTGCAGAGCCCAT
  TGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCAAATGGGGATGCTGATGAGCCAGATGGTGCA
  GGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAGATGCCG
  ACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGAAGAACC
  ATACTATGACTGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTGAAAACT
  CATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGCTACATCGAACGTGCCA
  GCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTCAAATGTGACGTCTGTGGGCAGCTCTT
  CAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCACACTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229077 representing NM_001146185
  Red=Cloning site Green=Tags(s)
  
  MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLS
  LPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLVQLAEDDGH
  SHMTQGHSSRSKRSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARESSD
  RSQRFPRMSDDNWKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSIHD
  QKGCPRKKPFECGSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMHTR
  ENLYEYGESFIHSVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAFMP
  SPTFSELQKIYGKDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPALNE
  FQKMYGKEKMYECKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKLCD
  FTDGRDAFMQSSELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISSNP
  YENQKIPTKENVYEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNTSE
  GREYSRSVIHSLVASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDSVI
  QSVFRAKPQKSVPGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRG
  MLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDF
  RQFFATSEDLNTNQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDDKI
  YECEDCGLGFVDLTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIHSS
  FLFEHQRIHEQDQLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHMRL
  HREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYT
  HTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLR
  IQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGA
  GIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKT
  HASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146185
• ORF Size: 4386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146185.1, NP_001139657.1
• RefSeq Size: 8375 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5178
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 166 kDa
• Gene Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'