PEG3 (NM_001146185) Human Tagged ORF Clone

CAT#: RG229077

PEG3 (GFP-tagged) - Human paternally expressed 3 (PEG3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PEG3
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229077 representing NM_001146185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGCGACGACGACATGACCCGGAACAGAAGAG
  AGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGGTGACCGGGACTGGGACCGGAGGGGCAGAAGCAG
  AGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAGAAGCAGGATGCCTCCGCGGGATCTT
  TCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAGGACGACAGGGACTCCAGGGCTTATG
  AGTCCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACCTCGCTGAGGACAGGAAACCTCACAA
  CACAATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCTCGGAGTGCAGCTTGCTGAAGACGAT
  GGCCACTCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGTGCCTACCCAAGCACCAGTCGAG
  GTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGATTTGTGAAGATGAATCTTCCCA
  CGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCCAAATCGGGAAGAGCAAGGGAGTCA
  AGCGACCGGTCACAGAGATTCCCCAGAATGTCAGATGATAACTGGAAGGACATTTCATTGAACAAGAGGG
  AGTCAGTGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTAGGGGAGGCTTTAGGTTTAATTCAACCCT
  TGTTTCCAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGACACAGATGGGAAGGGCTCGATT
  CACGATCAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGTGAGATGAGAAAAGCCATGAGCG
  TGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGCCAATTGATTTTGGGGCAATGCC
  ATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAATTTGTTGAGCACCAGATCATGCAT
  ACTAGAGAGAACCTCTATGAGTATGGTGAGTCCTTTATCCACAGTGTGGCTGTCAGTGAAGTTCAGAAAA
  GTCAGGTTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAGAGACCTTCAATAAGAGTGCCGCCTTGGC
  TGAACATCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAATCAGGAATGTGAGGAAGCCTTC
  ATGCCTAGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGACAAATTCTACGAGTGCAGGGTGT
  GTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAATCCACTTTGGGGATGACAAAGA
  TAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAAACCTTTAGGCCCAGCCCAGCCCTT
  AATGAGTTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAATGTAAGGTGTGTGGGGAGACTTTCCTTC
  ATAGCTCATCCCTGAAAGAACATCAGAAAATCCATACTAGAGGGAACCCATTTGAAAACAAGGGTAAAGT
  GTGTGAGGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAAAACTTACAATAAGGAGAAGCTC
  TGTGACTTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTCAGTGAGCATCAGAAAATTCATT
  CTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTCATAGTGGGCCATTCACTGAATC
  TCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGACGAAAAGGCGTTCACCATTAGCTCT
  AACCCCTATGAAAACCAGAAGATTCCCACTAAGGAAAATGTCTACGAGGCAAAATCATATGAGAGGTCTG
  TTATTCATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACAGTGTAGCAGGGCCCAGTAAACCAAAAGT
  AATGGCAGAGTCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAGAGTTCGTGCTGGAGGGAACACC
  TCTGAAGGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCTTCCAAACCTCCAAGAAGTCACA
  ATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTTATATCTCAGACCTTAATGATAA
  GCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGTAAGAATCGCAACTATGAAGACTCT
  GTCATACAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTTCCTGGAGAGGGATCTGGTGAGTTTAAGA
  AGGATGGCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAATACCAGAAGGCTCGTGCTAAAAAGAAATA
  CATTGAGCATAGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTTTGGTGAACAAACATTTCGCCCT
  CGAGGGATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCATAGCTCTGACCTCACTGAGCACC
  AGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAATGGTCTGTCATTCGCAGCTTGGC
  CCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAAGAGCAAGCGCGGAACAAATGTAAG
  GACTTCAGACAATTTTTTGCTACCAGCGAAGACCTCAACACAAACCAGAAAATCTATGACCAAGAGAAGT
  CTCATGGCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGGAGACCCACAGCGAGGAGACCCATGGTCA
  GGAGACAATTGAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCCTCAGAAGGATGACCCTGATGAC
  AAAATCTATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACAGACCTCACAGACCATCAGAAAG
  TCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTGTAATTCACACCCATTCCATCAG
  CGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCAAAGTGTGGGGAATCTTTTATTCAT
  AGCTCATTCCTTTTCGAGCATCAGAGAATCCATGAACAAGACCAGTTGTATTCCATGAAGGGGTGTGATG
  ATGGTTTTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATCGTGCTGCAGAGAGGAATCCTGCTCTTGC
  TGGGTCGGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAGCTCTGCCCTTAATGAGCATATG
  AGACTTCATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAGGAAGCTATCATTCCAGGCTTAG
  CCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAATGTGCAGTCTGTGGAGAATCTTT
  CGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAATGAGCCCTATGAGTACGGGTCCTCC
  TATACTCACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCTATACCATTCTATGAATGCAAGGATTGTG
  GTAAGTCCTTTATTCATAGCACAGTCCTCACTAAACATAAGGAGCTTCATCTGGAAGAAGAAGAAGAAGA
  TGAAGCAGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAATGTCCATGTTCCACAAGTAGTT
  CTGAGGATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAGGCTGCCGAGCCAGAAGTGGAGG
  CTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAGGGCCAGATGGAGAGGCTGCAGA
  GCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCAAATGGGGATGCTGATGAGCCAGAT
  GGTGCAGGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCAGAGGGAAAAGCTGAAGAGCCAGAGGGAG
  ATGCCGACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAGAAGGTGAAGATCAAGAGATTCAGGTAGA
  AGAACCATACTATGACTGCCATGAATGCACAGAAACCTTCACTTCCAGCACAGCATTCAGTGAACACCTG
  AAAACTCATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGGGAGTGCTCAGGCTACATCGAAC
  GTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACTTCAAATGTGACGTCTGTGGGCA
  GCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCACACTGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229077 representing NM_001146185
  Red=Cloning site Green=Tags(s)
  
  MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSGDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDL
  SLPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLGVQLAEDD
  GHSHMTQGHSSRSKRSAYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARES
  SDRSQRFPRMSDDNWKDISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSI
  HDQKGCPRKKPFECGSEMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMH
  TRENLYEYGESFIHSVAVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAF
  MPSPTFSELQKIYGKDKFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPAL
  NEFQKMYGKEKMYECKVCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKL
  CDFTDGRDAFMQSSELSEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISS
  NPYENQKIPTKENVYEAKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNT
  SEGREYSRSVIHSLVASKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDS
  VIQSVFRAKPQKSVPGEGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRP
  RGMLYECQECGECFAHSSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCK
  DFRQFFATSEDLNTNQKIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDD
  KIYECEDCGLGFVDLTDLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIH
  SSFLFEHQRIHEQDQLYSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHM
  RLHREDDLLEQSQMAEEAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSS
  YTHTSFLTEPLKGAIPFYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVV
  LRIQGLNVEAAEPEVEAAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPD
  GAGIEDPEERAEEPEGKAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHL
  KTHASMIIFEPANAFGECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146185
• ORF Size: 4386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146185.1, NP_001139657.1
• RefSeq Size: 8375 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5178
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• Gene Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'