FTSJ1 (NM_001282157) Human Untagged Clone

CAT#: SC334350

FTSJ1 (untagged) - Human FtsJ RNA methyltransferase homolog 1 (E. coli) (FTSJ1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FTSJ1
• Synonyms: CDLIV; JM23; MRX9; MRX44; SPB1; TRMT7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334350 representing NM_001282157.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTCTGAACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATATTCCGAGGC
  CGGGATGTGACGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGG
  AGCAGCCGGAACTCTAGCATCGAGGCCTTCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATC
  CCGGACCTGAGCAAACCCCTGCTGGACCATTCTTACGACCCAGATTTCAACCAGCTGGATGGTCCCACC
  CGCATCATTGTGCCTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTG
  GACCTAGAGGGCGGCTCAGAGTACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAG
  GAGGCCTGCACGTTGAAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCCAGGACTGCCCCATCAGC
  AGAGTGGACACGTTTCCCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAA
  GACAATGAAATGAGTTGTTCACCTTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282157
• Insert Size: 579 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282157.1
• RefSeq Size: 1669 bp
• RefSeq ORF: 579 bp
• Locus ID: 24140
• UniProt ID: Q9UET6
• Cytogenetics: Xp11.23
• MW: 21.2 kDa
• Gene Summary: This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (4) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter, compared to isoform a.