Glutathione S Transferase theta 1 (GSTT1) (NM_001293807) Human Untagged Clone

CAT#: SC334612

GSTT1 (untagged) - Human glutathione S-transferase theta 1 (GSTT1), transcript variant 2


  "NM_001293807" in other vectors (1)

Reconstitution Protocol

USD 477.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (2)
Rabbit polyclonal antibody to GSTT1 (glutathione S-transferase theta 1)
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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GSTT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC334612 representing NM_001293807.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGCCTGGAGCTGTACCTGGACCTGCTGTCCCAGCCCTGCCGCGCTGTTTACATCTTTGCCAAGAAG
AACGACATTCCCTTCGAGCTGCGCATCGTGGATCTGATTAAAGACGGAGTCTCGCTCTGTCGCCCAGGC
TGGAGTGCAGTGGCAGGATCACAGCTCACTGCAACCTCTGCCGCCCGAAACCTTGTCCTCTGCCTCTTG
TTCCTGCTGGGGAGGTCAGCACTTAAGCGATGCCTTTGCCCAGGTGAACCCCCTCAAGAAGGTGCCAGC
CTTGAAGGACGGGGACTTCACCTTGACGGAGAGGTGATGTTCCCTGTTTTCCTGGGTGAGCCAGTATCT
CCCCAGACACTGGCAGCCACCCTGGCAGAGTTGGATGTGACCCTGCAGTTGCTCGAGGACAAGTTCCTC
CAGAACAAGGCCTTCCTTACTGGTCCTCACATCTCCTTAGCTGACCTCGTAGCCATCACGGAGCTGATG
CATCCCGTGGGTGCTGGCTGCCAAGTCTTCGAAGGCCGACCCAAGCTGGCCACATGGCGGCAGCGCGTG
GAGGCAGCAGTGGGGGAGGACCTCTTCCAGGAGGCCCATGAGGTCATTCTGAAGGCCAAGGACTTCCCA
CCTGCAGACCCCACCATAAAGCAGAAGCTGATGCCCTGGGTGCTGGCCATGATCCGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI      Plasmid Map     
ACCN NM_001293807
Insert Size 681 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001293807.1
RefSeq Size 1067 bp
RefSeq ORF 681 bp
Locus ID 2952
UniProt ID P30711
Cytogenetics 22q11.23
Protein Pathways Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
MW 24.7 kDa
Gene Summary The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Transcript Variant: This variant (2) contains an additional exon but lacks a different exon, which results in a frameshifted segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.