Centaurin alpha 1 (ADAP1) (NM_001284311) Human Untagged Clone

CAT#: SC335024

ADAP1 (untagged) - Human ArfGAP with dual PH domains 1 (ADAP1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAP1
• Synonyms: CENTA1; GCS1L; p42IP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335024 representing NM_001284311.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTATTGGCTTCACTCGAGCTCCTTCGAGAGCAGTGGATCCGGGCCAAGTACGAGCGACAGGAGTTC
  ATCTACCCGGAGAAGCAGGAGCCCTACTCGGCAGGGTACCGTGAGGGTTTTCTCTGGAAGCGTGGCCGG
  GACAACGGGCAGTTTTTGAGCCGGAAGTTTGTGCTGACAGAACGAGAGGGTGCTCTGAAGTATTTCAAC
  AGAAATGATGCCAAGGAGCCCAAGGCCGTGATGAAGATCGAGCACCTGAACGCCACCTTCCAGCCGGCC
  AAGATCGGCCACCCCCACGGCCTGCAGGTCACCTACCTGAAGGACAACAGCACCCGTAACATCTTCATC
  TACCATGAGGACGGGAAGGAGATTGTGGACTGGTTCAATGCACTCCGAGCTGCTCGCTTCCACTACCTG
  CAGGTGGCATTCCCAGGGGCCAGCGACGCAGATCTGGTGCCAAAGCTCTCCAGGAACTACCTGAAGGAA
  GGCTACATGGAGAAGACGGGGCCCAAGCAAACGGAAGGCTTCCGGAAGCGCTGGTTCACCATGGATGAC
  CGCAGGCTCATGTACTTCAAAGACCCCCTGGACGCCTTCGCCCGAGGGGAAGTCTTCATTGGCAGCAAG
  GAGAGTGGCTACACGGTGCTGCATGGGTTCCCGCCGTCCACCCAGGGCCACCACTGGCCACATGGCATC
  ACCATCGTCACGCCCGACCGCAAGTTTCTGTTTGCCTGCGAGACGGAGTCCGACCAGAGGGAGTGGGTG
  GCGGCCTTCCAGAAGGCGGTGGACAGGCCCATGCTGCCCCAGGAGTACGCAGTGGAGGCGCACTTCAAG
  CATAAACCTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001284311
• Insert Size: 840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284311.1
• RefSeq Size: 1963 bp
• RefSeq ORF: 840 bp
• Locus ID: 11033
• UniProt ID: O75689
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome
• MW: 32.8 kDa
• Gene Summary: GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks 2 exons at the 5' end and contains an alternate 5' terminal exon, which results in translation initiation from an alternate start codon compared to variant 1. The resulting shorter isoform (4) has a distinct N-terminus compared to isoform 1.