Heme oxygenase 2 (HMOX2) (NM_001286271) Human Untagged Clone

CAT#: SC335103

HMOX2 (untagged) - Human heme oxygenase (decycling) 2 (HMOX2), transcript variant 9

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMOX2
• Synonyms: HO-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335103 representing NM_001286271.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTGACCTCTCGGAGCTCCTGAAGGAAGGGACCAAGGAAGCACACGACCGGGCAGAAAACACCCAG
  TTTGTCAAGGACTTCTTGAAAGGCAACATTAAGAAGGAGCTGTTTAAGCTGGCCACCACGGCACTTTAC
  TTCACATACTCAGCCCTCGAGGAGGAAATGGAGCGCAACAAGGACCATCCAGCCTTTGCCCCTTTGTAC
  TTCCCCATGGAGCTGCACCGGAAGGAGGCGCTGACCAAGGACATGGAGTATTTCTTTGGTGAAAACTGG
  GAGGAGCAGGTGCAGTGCCCCAAGGCTGCCCAGAAGTACGTGGAGCGGATCCACTACATAGGGCAGAAC
  GAGCCGGAGCTACTGGTGGCCCATGCATACACCCGCTACATGGGGGATCTCTCGGGGGGCCAGGTGCTG
  AAGAAGGTGGCCCAGCGAGCACTGAAACTCCCCAGCACAGGGGAAGGGACCCAGTTCTACCTGTTTGAG
  AATGTGGACAATGCCCAGCAGTTCAAGCAGCTCTACCGGGCCAGGATGAACGCCCTGGACCTGAACATG
  AAGACCAAAGAGAGGATCGTGGAGGAGGCCAACAAGGCTTTTGAGTATAACATGCAGATATTCAATGAA
  CTGGACCAGGCCGGCTCCACACTGGCCAGAGAGACCTTGGAGGATGGGTTCCCTGTACACGATGGGAAA
  GGAGACATGCGTAAATGCCCTTTCTACGCTGCTGAACAAGACAAAGGTGCCCTGGAGGGCAGCAGCTGT
  CCCTTCCGAACAGCTATGGCTGTGCTGAGGAAGCCCAGCCTCCAGTTCATCCTGGCCGCTGGTGTGGCC
  CTAGCTGCTGGACTCTTGGCCTGGTACTACATGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001286271
• Insert Size: 864 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286271.1
• RefSeq Size: 1627 bp
• RefSeq ORF: 864 bp
• Locus ID: 3163
• UniProt ID: P30519
• Cytogenetics: 16p13.3
• Protein Families: Transmembrane
• Protein Pathways: Porphyrin and chlorophyll metabolism
• MW: 32.8 kDa
• Gene Summary: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
  Transcript Variant: This variant (9) differs in the 5' UTR and coding sequence compared to variant 5. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.