1B (NC_009887) Virus Tagged ORF Clone

CAT#: VC100669

Myc-DDK-tagged ORF clone of viral ORF for 1B [Human enterovirus 100], codon optimized for human cell expression, YP_001497164

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100669 represents NCBI reference of YP_001497164 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCTAAGGCAGAGGAGTGTGGATACAGCGATAGAGTGAGAAGTATCACATTGGGCAACTCAACAA
  TCACTACTCAGGAGTGCGCCAATGTGGTGGTGGGGTACGGTGTTTGGCCATCTTATCTGGACGACGACGA
  GGCAACCGCGGAGGATCAGCCAACTCAACCCGACGTAGCCACATGCAGATTTTACACCCTTGAAAGTGTG
  CAATGGGAAACCGGATCAGACGGCTGGTGGTGGAAGTTCCCAGACATGCTGAAAAATATGGGACTCTTTG
  GCCAAAACATGCATTACCATTACCTTGGAAGAAGCGGCTACACTATTCACGTTCAATGCAATGCAAGCAA
  ATTCCACCAGGGATGTGTGCTGGTGGTTTGCGTCCCCGAGGCGGAGATGGGTTGTGCGCAAACTGACTCT
  ACGTTCGAACATACAGAGCTTACAAAACAGGAGGAGCCATTTAAGTTTACTTCTACCCCGACAAGCGGAA
  AAGTGGTACAAGCCGCCGTGTGTAATGCCGCTATGGGGGTGGGCGTGGGCAACCTGACCATATTCCCACA
  CCAGTGGATCAACCTGAGAACTAATAATTCAGCTACAATAGTGATGCCTTATATAAATTCAGTTCCAATG
  GATAACATGTTTAGACACTATAACTTCACCCTTATGATCATCCCATTCGCACCCCTGAAGTATCAACCCG
  GTGCCACTCCGTACGTCCCAATCACGGTGACCGTTGCCCCGATGTGTGCAGAATACAATGGGCTCCGCCT
  CGCATCTAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100669 representing YP_001497164
  Red=Cloning sites Green=Tags
  
  MSPKAEECGYSDRVRSITLGNSTITTQECANVVVGYGVWPSYLDDDEATAEDQPTQPDVATCRFYTLESV
  QWETGSDGWWWKFPDMLKNMGLFGQNMHYHYLGRSGYTIHVQCNASKFHQGCVLVVCVPEAEMGCAQTDS
  TFEHTELTKQEEPFKFTSTPTSGKVVQAAVCNAAMGVGVGNLTIFPHQWINLRTNNSATIVMPYINSVPM
  DNMFRHYNFTLMIIPFAPLKYQPGATPYVPITVTVAPMCAEYNGLRLASKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009887
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009887.1, YP_001497164
• RefSeq ORF: 783
• MW: 29.1 kDa