2C (NC_009887) Virus Tagged ORF Clone

CAT#: VC100674

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 100], codon optimized for human cell expression, YP_001497169

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100674 represents NCBI reference of YP_001497169 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAATGGTTGGCTGAAGAAATTCACAGAAATGACCAACGCTTGTAAGGGAATGGAATGGATCGCCG
  TGAAAATTCAGAAGTTCATTGAGTGGTTGAAGGTCAAAATTCTTCCCGAGGTGAAGGAGAAGCATGAGTT
  TCTGAACCGCCTGAAGCAGCTTCCACTGCTGGAAAGTCAGATTGCTACTATTGAACAAAGCGCACCTAGC
  CAATCCGACCAGGAGCAACTCTTCTCTAATGTGCAGTACTTTGCACATTACTGCCGGAAGTACGCCCCAC
  TGTATGCTGCCGAGGCAAAGAGAGTTTTCAGCCTCGAAAAAAAGATGTCAAATTACATTCAGTTCAAATC
  TAAGTGTCGGATCGAACCTGTTTGTCTTCTCCTCCACGGATGCCCGGGAGCTGGCAAGAGCGTCGCCACC
  AATCTCATAGGAAGGAGCCTGGCTGAGAAACTGAATTCATCCGTTTATAGTTTGCCACCCGATCCCGATC
  ATTTCGATGGGTACAAGCAGCAGGCCGTAGTAATCATGGATGACCTGTGCCAGAATCCAGATGGGAAGGA
  CGTGTCCCTTTTTTGTCAGATGGTATCCAGCGTTGACTTCGTTCCCCCTATGGCTGCTTTGGAGGAGAAA
  GGGATCCTGTTTACGTCCCCATTTGTCCTTGCAAGCACCAACGCTGGGTCAATCAATGCCCCCACCGTGA
  GTGATTCTAGGGCACTGGCGAGACGCTTTCATTTCGATATGAATATTGAGGTGATTAGTATGTACTCCCA
  GAACGGTAAAATTAATATGCCCATGTCAGTCAAGACCTGCGACGAGGAATGCTGTCCCGCTAATTTTAAG
  AAGTGCTGCCCTCTGGTGTGTGGCAAAGCCATCCAGTTTATTGATCGCCGGACGCAGGTGAGGTACTCAC
  TCGACATGCTCGTGACGGAAATGTTTCGAGAGTATAACCACCGCCATAGTGTGGGGGCCACCCTGGAAGC
  CTTGTTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100674 representing YP_001497169
  Red=Cloning sites Green=Tags
  
  MSNGWLKKFTEMTNACKGMEWIAVKIQKFIEWLKVKILPEVKEKHEFLNRLKQLPLLESQIATIEQSAPS
  QSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGCPGAGKSVAT
  NLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEK
  GILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVKTCDEECCPANFK
  KCCPLVCGKAIQFIDRRTQVRYSLDMLVTEMFREYNHRHSVGATLEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009887
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009887.1, YP_001497169
• RefSeq ORF: 990
• MW: 37.4 kDa