1D (NC_009887) Virus Tagged ORF Clone

CAT#: VC100671

Myc-DDK-tagged ORF clone of viral ORF for 1D [Human enterovirus 100], codon optimized for human cell expression, YP_001497166

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100671 represents NCBI reference of YP_001497166 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACACCGATCAACGAAATGGGCAGGTGGTGGACACCAAGGCTACAAATCCCACCAACAGCACAG
  CCGTTCCAGCACTCACAGCGGTAGAGACCGGCCACACAAGTCAGGTGGTGCCCGGCGATACAATACAGAC
  CCGACACGTGGTGAATTTCCATAGCCGGAGCGAGTCTAGCATCGAGAACTTTATGGGCAGGTCAGCATGC
  GTATTCATGAAGACCTATAAGGCGATGGGCGAAGATACAGACACAAGCCGATTTGCCGCGTGGGACATCA
  ACATTCGCGAAATGGTGCAGTTGCGGAGGAAGTGCGAAATGTTCACCTATCTGAGATTCGATATTGAGGT
  TACATTCGTCATCACAAGCTGTCAGGACCCAAGCACACAGCTGGATCAGGATATGCCAGTACTTACACAC
  CAAATTATGTATATTCCACCTGGCGGACCAATTCCCGACAAAGTGAACAGTTACGCCTGGCAGACTAGCA
  CCAACCCATCCGTTTTTTGGACTGAAGGGAACGCACCCGCGAGAATGTCTATCCCGTTCATTTCCGTGGG
  CAACGCCTACTCAACCTTCTACGACGGGTGGTCTCACTTTTCTCAGAGCGGAGTTTACGGCTACACGACC
  CTGAACGCCATGGGGAAGCTGTATGTGCGACACGTCAACGCAAAGTCACCAACTCAGATTACCTCCACCA
  TCCGGGTGTACTTCAAACCTAAGCACGTGAAGGCGTGGGTCCCAAGACCACCAAGGCTCTGCCCCTACCT
  GAGGAAAGCGTCTGTCAACTTCGACGTCACCAACATAACTGAAGAAAGGACGACTATTAATAAAGTGCCC
  ACTGAGGACCATTCAGCTCTGAAGAATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100671 representing YP_001497166
  Red=Cloning sites Green=Tags
  
  MGDTDQRNGQVVDTKATNPTNSTAVPALTAVETGHTSQVVPGDTIQTRHVVNFHSRSESSIENFMGRSAC
  VFMKTYKAMGEDTDTSRFAAWDINIREMVQLRRKCEMFTYLRFDIEVTFVITSCQDPSTQLDQDMPVLTH
  QIMYIPPGGPIPDKVNSYAWQTSTNPSVFWTEGNAPARMSIPFISVGNAYSTFYDGWSHFSQSGVYGYTT
  LNAMGKLYVRHVNAKSPTQITSTIRVYFKPKHVKAWVPRPPRLCPYLRKASVNFDVTNITEERTTINKVP
  TEDHSALKNY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009887
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009887.1, YP_001497166
• RefSeq ORF: 870
• MW: 32.7 kDa