1C (NC_009887) Virus Tagged ORF Clone

CAT#: VC100670

Myc-DDK-tagged ORF clone of viral ORF for 1C [Human enterovirus 100], codon optimized for human cell expression, YP_001497165

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100670 represents NCBI reference of YP_001497165 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTGCCAGCTCTGCTGACTCCCGGTAGCAACCAGTTCCTCACATCTGATGATTTCCAGAGTCCAT
  CCGCGATGCCCCAGTTTGATCCAACCCCAGAGATGCGGATTCCCGGGGAAGTAAAAAACCTTATGGAGAT
  CGCTGAAGTCGACTCAGTTATGCCCGTTAATAACGTACCTGGCAACTGCAATACCATCCGCGCTTACCAG
  ATCCCAGTGCAGGCCAATAATGCTAACACCACAACACCCATCTTTTCCTTCCAGGTGGATCCCGGCTACT
  CCTCCGTGCTGAACAGGACACTGCTGGGCGAAATGCTGAATTATTATGCTCACTGGAGCGGCTCTATAAA
  GCTCACCTTTCTGTTCTGTGGTAGTGCCATGGCTACAGGGAAGCTTCTGTTGGCTTATTCCCCCCCCGGG
  GCCTCTGTGCCAGAGTCTAGGAAAGATGCAATGCTGGGAACGCATATTATCTGGGACCTGGGCCTCCAGT
  CTAGCTGTGTCCTGTGTGTTCCCTGGATCTCCCAGAGTCACTACCGGATGGTGCACAAAGATACCTACAC
  CCTGGCAGGCTACATCACTTGCTGGTACCAGACCAACATCGTTGTGCCCCCTGAAACACCTGCAGACTGT
  ACAATCCTGGGCTTTGCGTCCGCCTGCAACGACTTCTCCGTTCGCCTCCTCAGAGATACGCCATTTATCT
  CACAGACGACCGATCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100670 representing YP_001497165
  Red=Cloning sites Green=Tags
  
  MGLPALLTPGSNQFLTSDDFQSPSAMPQFDPTPEMRIPGEVKNLMEIAEVDSVMPVNNVPGNCNTIRAYQ
  IPVQANNANTTTPIFSFQVDPGYSSVLNRTLLGEMLNYYAHWSGSIKLTFLFCGSAMATGKLLLAYSPPG
  ASVPESRKDAMLGTHIIWDLGLQSSCVLCVPWISQSHYRMVHKDTYTLAGYITCWYQTNIVVPPETPADC
  TILGFASACNDFSVRLLRDTPFISQTTDLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009887
• ORF Size: 720 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009887.1, YP_001497165
• RefSeq ORF: 720
• MW: 26.4 kDa