E7 protein (NC_008189) Virus Tagged ORF Clone

CAT#: VC101769

Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus 101], codon optimized for human cell expression, YP_656499

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101769 represents NCBI reference of YP_656499 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGGCAGAAGGCCACCATCGGCGACGTAAAGCTCGAGCTGAGCCCTCTGGTGCTTCCTGCAAATC
  TGCTCTGTGACGAGCTGGTGACGGAGGAGGAAGAACAGCAGGATGAGCAAGCTCTCTCCCCCTACAGAAT
  AGTGACCGACTGTGGCCAATGCCATAAAACCCTGGCACTCCATGTACTGGCCACCGACGGGGCAATTCGA
  TCCGTCGAGCAGCTGCTGTGTGGTGATCTTGAGTTGGTGTGTGCAAGCTGTATCCGAGCTCAGAGACATG
  GGGGTCAGAGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101769 representing YP_656499
  Red=Cloning sites Green=Tags
  
  MRGQKATIGDVKLELSPLVLPANLLCDELVTEEEEQQDEQALSPYRIVTDCGQCHKTLALHVLATDGAIR
  SVEQLLCGDLELVCASCIRAQRHGGQRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_008189
• ORF Size: 294 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_008189.1, YP_656499
• RefSeq ORF: 294
• Locus ID: 5177022
• MW: 10.7 kDa