E4 protein (NC_008189) Virus Tagged ORF Clone

CAT#: VC101772

Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus 101], codon optimized for human cell expression, YP_656502

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101772 represents NCBI reference of YP_656502 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCCCCGACACTACCCGCGGGGGGCTCGGCGATCTGCTCAAGGACCCCGATCGACCCCCAGCCC
  ACCCCCGCCACCGCAGACCTAAAGCCATTCCCCTTGGAACAATCCCCGAAGCAGATGAATCCAATAAAGA
  GAACATCAATCCGAACGACCCTGGAGGCGACCCCTCCCTGCTGAGTTCTTTGCTGCACCAGTGGGGGAAG
  GATATCGATTGTCTTATTGACACCATCGTTAGGGATCTGCAAGACTATAAGACACGCTTGGGCATCCGGC
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101772 representing YP_656502
  Red=Cloning sites Green=Tags
  
  MKLPDTTRGGLGDLLKDPDRPPAHPRHRRPKAIPLGTIPEADESNKENINPNDPGGDPSLLSSLLHQWGK
  DIDCLIDTIVRDLQDYKTRLGIRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_008189
• ORF Size: 282 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_008189.1, YP_656502
• RefSeq ORF: 282
• Locus ID: 5177019
• MW: 10.5 kDa