E1 (NC_008189) Virus Tagged ORF Clone

CAT#: VC101770

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 101], codon optimized for human cell expression, YP_656500

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101770 represents NCBI reference of YP_656500 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATAAGGATAACAAGGGCACCTCCCCTCACATTTGTGACTATAATGCCATTGACTTTATTGATG
  CCGAGGCCGCCTGCGAAGAACTCGATCCTTTGGAGGCCGTCCTGGAAGAGTCAGACGGCACCGATATAAG
  TGACCTGATTGACAATACAGAAGAACCAGACGAGGGCAATAGTCTGGCTTTGTTGAATAAGCAGCAGCTC
  GAGGATGATACCCAACAGCTGAAGATTCTTAAACGCAAATATTTCTCTCCCAGCCCCAAGCAGCAGGCAG
  TGATTGATCTCTCACCCCGCCTGCAGCAGTTGCGGGTCTCTAGTCAAGGGTCTTCCAAGAGGAGGCTGGT
  ATTCGAGGATAGCGGACTCGGTAACGAGACTGAGGATTCCGTCACTGAAGTGGCCAAGGTTGCCTCTGAG
  CAGCCACTGAGCTGTAACCCCGGCGAACCAGAGACAAACGAAGACGTGAGTACGGACGTAGAGACTCGGT
  CAGGAGGCCCTTGCGGCGGCACAACAACCAGTAACAATAACTCCAACAACAACTGCATCGACCTGCTCCA
  TTCATCAAACAGGGAAGCCACTGCCTACGCCAAATTCAAGGCCACCTTTGATGTGGGATATAAGGAGCTG
  ACACGACCGTTCATATCCAACAAATCCTGCTGCTGTTCCTGGATCGCTGGTATCTTTGGCGTCGTTGCCG
  AAATACTCGAGGCGGCCAAGACACTGCTGCAGCCACATTGCGAATATCTGCAAATAATCAATCCTTCCAT
  GGGAACAGGGGTCACCGTGATGATGCTCTTCCAGTTCTACGCAGCCAAGTGTCGGGATACTGTGATCAAT
  CTGCTGTGCAACGTTCTTCATGTCAGAGAGTGGCAGATCATCACCAATCCACCCAAACACCGCAGTGTGG
  CAGTCGCTCTGTACTTCTACAAGACGAGTATGTCTAACGTCAGCTATTGCTACGGCGCAATGCCAGAGTG
  GATCAAAAAACAAACGCTTGTGAATCATCAGCAAGAAAGCGATAATTTTGAGTTTGCAACCATGGTGCAG
  TGGGCCTATGATAATAACATCCGCGATGAAGCAGAGGTTGCATACGGTTACGCCTCACTGGCCGATGATG
  ACACTAACGCTGCCGCCTGGCTGAAGTGTAACAATCAATTTAAGTACGTGAAAGATTGTGTGCAAATGGT
  TGCTATGTACAAGAGGTACGAGATGCGGAACATGACAATCGGGCAGTGGCTCGTGAAATGCGGCGAGCGA
  GTTACTGAGGAAGGAAATTGGAAAAACATCATCAACTTCTTGAAATACCAAGAGATTTCCATCGTGGCAT
  TTCTCACTACTCTCAGATATTTCTTGCAGGGGAGACCGAAGAAAAACTGTCTCGCTATCTGGGGGCCACC
  TGACACCGGTAAATCTATGTTCTGTTATTCCCTGATTAAATATACTCAGGGCAAGGTCGTTTCTTTTGTA
  AACAGCCGGTCCCAGTTCTGGCTGCAGCCCCTGGTGGACGGAAAAATTGGACTCATTGATGACGCTACAT
  TCGCTTGCTATCAGTATATGGATGTATATATGCGGAACGGTTTGGATGGCAACGCCGTGAGTGTCGACGT
  CAAACACAAGGCTCCCATCCAGCTGAAACTGCCTCCCCTTCTGCTTACATCTAATATCGAAGTCCACGCC
  GAGGCCCCGCTGAAGTACCTCCACAGTCGCATACAGGAGTATAAGTTCCCAAACAAACTCAGGCTGGATG
  CAAACGGGAATCCGATCATTACGATAACCGACGCTGATTGGAAAAGTTTCTTCTCAAAGCTGTGGAAGCA
  ACTGGACCTCGGAGATCCTGATGACAGTTCTGACGGCGATCCGGGCAGGCCCTTCCGGTGTTGCGCACGG
  CAGGCCACTGGAGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101770 representing YP_656500
  Red=Cloning sites Green=Tags
  
  MEDKDNKGTSPHICDYNAIDFIDAEAACEELDPLEAVLEESDGTDISDLIDNTEEPDEGNSLALLNKQQL
  EDDTQQLKILKRKYFSPSPKQQAVIDLSPRLQQLRVSSQGSSKRRLVFEDSGLGNETEDSVTEVAKVASE
  QPLSCNPGEPETNEDVSTDVETRSGGPCGGTTTSNNNSNNNCIDLLHSSNREATAYAKFKATFDVGYKEL
  TRPFISNKSCCCSWIAGIFGVVAEILEAAKTLLQPHCEYLQIINPSMGTGVTVMMLFQFYAAKCRDTVIN
  LLCNVLHVREWQIITNPPKHRSVAVALYFYKTSMSNVSYCYGAMPEWIKKQTLVNHQQESDNFEFATMVQ
  WAYDNNIRDEAEVAYGYASLADDDTNAAAWLKCNNQFKYVKDCVQMVAMYKRYEMRNMTIGQWLVKCGER
  VTEEGNWKNIINFLKYQEISIVAFLTTLRYFLQGRPKKNCLAIWGPPDTGKSMFCYSLIKYTQGKVVSFV
  NSRSQFWLQPLVDGKIGLIDDATFACYQYMDVYMRNGLDGNAVSVDVKHKAPIQLKLPPLLLTSNIEVHA
  EAPLKYLHSRIQEYKFPNKLRLDANGNPIITITDADWKSFFSKLWKQLDLGDPDDSSDGDPGRPFRCCAR
  QATGDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_008189
• ORF Size: 1908 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_008189.1, YP_656500
• RefSeq ORF: 1908
• Locus ID: 5177020
• MW: 71.7 kDa