E2 protein (NC_008189) Virus Tagged ORF Clone

CAT#: VC101771

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 101], codon optimized for human cell expression, YP_656501

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101771 represents NCBI reference of YP_656501 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGTTGGTGGATCGGTTCGACGCTGTTCAGGACCGGCTGCTGGGAATTTATGAAGCTGGCCGGA
  CCGACATCGATGGTCAGATTGTGCACTGGGACCTTGTGAGACAAGAGTACGTACTGCTGCACTATGCACG
  AAAAAACGGCATTTCCAACCTGGGCATTCAGAAAGTGCCCTCCCTGGCTACATCTGAGTCTAAGGCGAAG
  CAGGCAATACTGATGGGGATCGTTCTGAAATCACTCAAGAACAGCGCATTCGGCAATGAGCAGTGGACTC
  TCACTGATACAAGCTACGAACTGTTCATGACACCCCCAAGCCATACCTTCAAGAAACAAGGGAAAACTAT
  TGAAGTGTGGTACGGCAATAACGAGGACAACGCAGTGCCATATACCCTCTGGACTGCCATCTACTATCAG
  GACGCTCAGGATAACTGGTACAAAGCAAGAGGACAGGTGTCTGCAGATGGACTGTACTATATCGATTGGG
  AGGGGAATAAACATTTCTACGTGCGATTCGCCGACGAGGCGACCCAATTCGACGCTGCCAACGAATGGCA
  AGTTAGAACCGAAAGCGAAATCATTAGCAATTCAGTCACCAGCACACCTCCCAGATCCGATCCTAGACCA
  ACCGGGAGCTCCGCTATTACATATGAGACCAGTCGGCACGACACACGCCGGACCCGGCGCAGCACCAAAA
  GACCCAGGCCATCACCTTCTCCGTCAGCGACCAGTGCCACGGAGTCCGACACCCCAGGACATCATACACG
  GTCCCGCCGAATCCAGCAACGGAAGCACAAAAGCCAGAGACCCCGCTGGGGAAGTGAATCCCCAATACAG
  AGCCCATCTTCAGTGGGACAGGGCCATCGCCTGCCTAACAGACATTATAGATCTCGGCTTGCTCGATTGC
  AGGATGAAGCCCGAGACCCTCCCGTCATCTTGATTAAAGGACCCGCAAACACTACCAAATGTTGGCGCTA
  TAGAATGAAACAGAATTATAGGGCACTTTTTGAATCTATTTCCACAGCCTTCTCCTGGGTGGGTGATCGG
  GGCTCCGACAGAGTGGGCTGTTCTAGGGTGCTCGTGGCCTTCCGAAACAATAGTCAGAGGGAGAGGTTCC
  TCCATACCGTCAAACTCCCAAAAGGCACCCAGTTTAGTTTTGGCAACCTCGAGAGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101771 representing YP_656501
  Red=Cloning sites Green=Tags
  
  METLVDRFDAVQDRLLGIYEAGRTDIDGQIVHWDLVRQEYVLLHYARKNGISNLGIQKVPSLATSESKAK
  QAILMGIVLKSLKNSAFGNEQWTLTDTSYELFMTPPSHTFKKQGKTIEVWYGNNEDNAVPYTLWTAIYYQ
  DAQDNWYKARGQVSADGLYYIDWEGNKHFYVRFADEATQFDAANEWQVRTESEIISNSVTSTPPRSDPRP
  TGSSAITYETSRHDTRRTRRSTKRPRPSPSPSATSATESDTPGHHTRSRRIQQRKHKSQRPRWGSESPIQ
  SPSSVGQGHRLPNRHYRSRLARLQDEARDPPVILIKGPANTTKCWRYRMKQNYRALFESISTAFSWVGDR
  GSDRVGCSRVLVAFRNNSQRERFLHTVKLPKGTQFSFGNLESL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_008189
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_008189.1, YP_656501
• RefSeq ORF: 1179
• Locus ID: 5177021
• MW: 45.1 kDa