E4 (NC_014952) Virus Tagged ORF Clone

CAT#: VC101859

Myc-DDK-tagged ORF clone of viral ORF for E4 [Human papillomavirus type 128], codon optimized for human cell expression, YP_004169267

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101859 represents NCBI reference of YP_004169267 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTACACCCTCATGACTTACCAGGGCACAGAGATAATATTCTGCTATTTTCTCCTGAACCTGAAAA
  ATATGGCCAAGCTCGACTACGGACTCTGCATAACGAACCTCCAGCTGTTCCCTCTGCTGCTCCCAGCTCA
  TCAGAATAGCCCTGCAGCAGGGACTCGCGCATCCCCCCCAACCTCACAAGGACCACACCAAGACGCCCCA
  CATCGCCGAAAAGAACATCTGGATGACGTTAGAAAACCTGGGCGACGGGACCCATTGACAACTCGGCCCC
  ACCTGAGATGGGATCTGGATGAGGAGAATGAAAAGGAAAACCAAAACCCTAACCTCCACCAGGAAGATCA
  GCAAGAAAACGAGGAGTATACACAGTCCTACCTGCAGAGCCTGCTGAAGAAGTGGGAAGGCGATATCGAT
  AGATTTAGGGATATGGTGTACAGAGACCTGGAAGGCCTTAAACAAAAACTGGGAATACAGATGTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101859 representing YP_004169267
  Red=Cloning sites Green=Tags
  
  MDYTLMTYQGTEIIFCYFLLNLKNMAKLDYGLCITNLQLFPLLLPAHQNSPAAGTRASPPTSQGPHQDAP
  HRRKEHLDDVRKPGRRDPLTTRPHLRWDLDEENEKENQNPNLHQEDQQENEEYTQSYLQSLLKKWEGDID
  RFRDMVYRDLEGLKQKLGIQMSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014952
• ORF Size: 489 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014952.1, YP_004169267
• RefSeq ORF: 489
• Locus ID: 10146723
• MW: 19.1 kDa