E7 (NC_014952) Virus Tagged ORF Clone

CAT#: VC101861

Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 128], codon optimized for human cell expression, YP_004169264

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101861 represents NCBI reference of YP_004169264 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGCAATACTTCAACGATTAGAGATATTGACCTCCACTTGGAGGAGCTGGTGCTGCCGGCCAACC
  TCTTGTCAACTGAGTCTTTGTCACCCGACGACGAGCTGGAAGAAGAACAGCGGCAGTCTTTTTCTGTCGA
  CACATGTTGCGATCACTGCAACCGAAGGGTCAGGTTGGTCGTGTCTGCTACAAGTACGGGCATCATACGG
  TTCCAACAGCTGCTGGTGAGTGAACTGAGTATCGTGTGCCCTCGGTGTTGTCGAGAAAATTTTCAGCACG
  GAAGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101861 representing YP_004169264
  Red=Cloning sites Green=Tags
  
  MRGNTSTIRDIDLHLEELVLPANLLSTESLSPDDELEEEQRQSFSVDTCCDHCNRRVRLVVSATSTGIIR
  FQQLLVSELSIVCPRCCRENFQHGRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014952
• ORF Size: 288 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014952.1, YP_004169264
• RefSeq ORF: 288
• Locus ID: 10146720
• MW: 10.9 kDa