L2 (NC_014952) Virus Tagged ORF Clone

CAT#: VC101863

Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 128], codon optimized for human cell expression, YP_004169268

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101863 represents NCBI reference of YP_004169268 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAGCCAAGGCGCAAGCGGGCCTCACCCGAAGATCTTTACAAGAGTTGCGCCTTGGGGGGTGACT
  GCTTCCCAGACGTGCAGAACAAGATTCAAGGTACAACTCTGGCAGACAGACTTCAGGTCTTTGGGAGTAT
  ACTTTACTTCGGTGGCCTGGGAATCGGATCCGGTAAAGGGACTGGTGGCGCGACAGGGTACCGACCGTTC
  GGCGGGGGCGGAACTGTTTCCCGCCCCTCCATACGAATTCAGCCGGAATCTCTGCCTAAGCCTTCAGTCC
  CTATCGATCCACTTGGAGGAGCTGAGCTCATTCCCTTGGACGTAATCGACGCAACCGCACCTTCCGTGGT
  GCCCCTGACTGAAGGGGTGCCTGAGACCACAATCATTAGCAGCGGAACCGGACCAACCATTACTGCAGAA
  GAACTGGATGTAACAACGCAGATCGACCTTAACACATTTAATAACATCAACGAGCATCCCGCAGTAATTA
  ACGTCACGGATGAAGGTACAACCGCACTGGATGTCCAGACTCTCCCACCCCCTAGCAAGAAACTTATCTT
  GGACTCCACCATTACTGATCAGATCGAACTGGTGCCTCTCCATAGAATCTATACAGATCAGAACGTGAAT
  ATGTTCGTGGACACCCACTTTGAAGGGCACATCGTAGGCGGGGATCCTAATTTTACAGATATAGTGCTGC
  GCGAACAGTTCGAGATAGAGGAGGTGAGTCCGAAGACAAGCACCCCGACCGAAAAACTTTCAACTGCCGT
  TGGCAAAGCCCGCCGCGTGTACAACCGGTTTGTAAAGCAGGTGCCCACGAGCAGCCTCGAGGCCGTGGTA
  AACCCATCCCGCCAGGTGATCTTTGAGATCGAGAATCCCGCATTTGATGATGAGGTAAGCCTTCTCTTCA
  ACCAGGAGCTGGCTGAGGTTACCGCCACACCCAATCCGGATTTCCGAGACATCAATTACCTCAGTCGACC
  TTTCTACTCCGAGACAGAGAGTGGCAGGGTTCAGTACAGCCGGCTGGGTCAGCGCGCTAGTATGCAGACC
  CGCTCCGGCCTGGTAATCGGAGAGAAGGTGCAGTTCACATATAATATTTCAGATATTGAACCAGTTGAGA
  CGATAGAGCTGAGTACCCTCGGCACAACTTCCGCCGATTCAATCATACAGAATGCCCAGGCTGAGAGCGT
  ATTCATCGATGCCCAGGACTCAACACCTCTCACCTATACAGAAGACCAGCTCTTGGACGAACCGAATGAA
  GACTTCGCCAATGCCCACCTGGTGCTCACGACTACGGACGACTTCGGCGATACCTACGACTATCCCGTTA
  TTCCCCCCGGCCTCGGGTACAAACTCCTGATCCCTAACTACAACGTAACCCTGTCCTCATTTCAGCTGCC
  AGAAGCTAGCGTTCCGACAATCCTTCCTGAGATCCCATTCGCCCCTTACGCTCCCTCCCCCATAACCGTA
  TTCGGCGATACTTTCTACTTGAACCCTAGCCTGCTGAAGCGAAAGCGGAAGCAGTATTTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101863 representing YP_004169268
  Red=Cloning sites Green=Tags
  
  MSKPRRKRASPEDLYKSCALGGDCFPDVQNKIQGTTLADRLQVFGSILYFGGLGIGSGKGTGGATGYRPF
  GGGGTVSRPSIRIQPESLPKPSVPIDPLGGAELIPLDVIDATAPSVVPLTEGVPETTIISSGTGPTITAE
  ELDVTTQIDLNTFNNINEHPAVINVTDEGTTALDVQTLPPPSKKLILDSTITDQIELVPLHRIYTDQNVN
  MFVDTHFEGHIVGGDPNFTDIVLREQFEIEEVSPKTSTPTEKLSTAVGKARRVYNRFVKQVPTSSLEAVV
  NPSRQVIFEIENPAFDDEVSLLFNQELAEVTATPNPDFRDINYLSRPFYSETESGRVQYSRLGQRASMQT
  RSGLVIGEKVQFTYNISDIEPVETIELSTLGTTSADSIIQNAQAESVFIDAQDSTPLTYTEDQLLDEPNE
  DFANAHLVLTTTDDFGDTYDYPVIPPGLGYKLLIPNYNVTLSSFQLPEASVPTILPEIPFAPYAPSPITV
  FGDTFYLNPSLLKRKRKQYFY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014952
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014952.1, YP_004169268
• RefSeq ORF: 1533
• Locus ID: 10146724
• MW: 55.9 kDa