E1 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101899

Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 16], codon optimized for human cell expression, NP_041327

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101899 represents NCBI reference of NP_041327 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATCCCGCAGGCACAAATGGGGAAGAGGGCACTGGATGTAATGGGTGGTTCTATGTCGAGGCCG
  TAGTGGAAAAAAAGACCGGCGATGCTATTTCAGACGACGAAAACGAGAATGACTCAGATACCGGCGAGGA
  CCTGGTCGATTTCATTGTGAACGACAACGATTACCTGACACAGGCTGAGACCGAGACAGCGCACGCTTTG
  TTCACCGCACAAGAGGCAAAACAGCATCGCGATGCCGTTCAGGTGCTGAAGCGAAAGTACCTTGTGAGCC
  CACTCAGCGATATCTCTGGCTGCGTGGATAATAATATCTCCCCGAGGCTGAAGGCAATTTGCATAGAGAA
  GCAGTCAAGGGCCGCGAAGCGACGGCTCTTTGAGAGCGAAGATTCCGGCTACGGCAACACCGAAGTCGAG
  ACCCAACAAATGCTGCAAGTCGAGGGACGACATGAGACCGAGACACCCTGTTCCCAGTACAGTGGAGGCT
  CAGGGGGCGGCTGCTCTCAATATTCTTCTGGATCAGGCGGGGAAGGGGTGTCAGAGAGGCACACAATCTG
  CCAAACCCCACTCACCAACATCCTGAATGTGCTCAAAACATCTAATGCAAAGGCGGCCATGCTGGCCAAG
  TTCAAGGAACTGTACGGAGTGTCATTTAGCGAGCTGGTGCGGCCCTTCAAGTCTAACAAAAGTACCTGTT
  GTGACTGGTGCATCGCTGCCTTTGGACTTACCCCCTCAATCGCGGACAGCATTAAAACACTCCTGCAGCA
  GTATTGCCTGTACTTGCATATCCAGTCATTGGCTTGTTCTTGGGGGATGGTTGTCCTGTTGCTTGTTCGG
  TACAAATGTGGCAAGAATCGCGAGACCATAGAGAAACTTCTGTCAAAGTTGCTGTGCGTGTCACCTATGT
  GCATGATGATCGAGCCCCCTAAGCTGCGAAGCACTGCAGCAGCCCTCTACTGGTACAAAACAGGTATCTC
  TAACATTAGTGAAGTCTATGGAGACACACCCGAGTGGATTCAGCGGCAAACTGTACTGCAGCATTCTTTC
  AATGACTGTACATTTGAACTCAGTCAAATGGTACAGTGGGCTTACGACAACGACATCGTTGATGATTCTG
  AAATAGCTTACAAATACGCCCAGCTGGCCGATACTAACAGCAACGCATCAGCTTTTTTGAAGAGCAACTC
  CCAAGCTAAGATTGTGAAGGACTGCGCCACTATGTGCAGGCACTATAAGCGCGCCGAAAAGAAACAAATG
  TCCATGTCTCAATGGATCAAATACAGATGTGATCGAGTCGACGATGGGGGCGACTGGAAACAGATCGTCA
  TGTTCCTGCGCTACCAGGGCGTCGAATTCATGTCCTTTCTCACCGCCCTCAAAAGATTTCTCCAGGGGAT
  CCCAAAGAAGAATTGTATTTTGTTGTATGGGGCCGCTAACACAGGCAAGTCCCTGTTCGGGATGTCCCTG
  ATGAAGTTTCTTCAAGGTAGTGTAATCTGCTTCGTGAACAGCAAATCCCACTTTTGGCTGCAGCCCCTTG
  CAGACGCTAAAATCGGTATGCTCGATGATGCAACAGTGCCATGTTGGAACTATATTGATGATAACTTGAG
  GAACGCACTCGATGGCAACCTGGTTTCCATGGACGTGAAGCACAGGCCGCTGGTTCAGCTGAAATGTCCG
  CCCCTGCTCATCACGAGCAATATCAATGCGGGAACTGATTCTCGGTGGCCTTACCTCCACAATCGGCTCG
  TGGTGTTCACATTCCCCAATGAGTTCCCCTTCGACGAAAATGGTAACCCTGTCTATGAACTGAATGACAA
  GAACTGGAAGTCCTTCTTTTCCCGCACCTGGAGCCGCCTGAGTCTGCACGAAGACGAGGATAAGGAGAAT
  GACGGCGACTCTCTGCCAACGTTTAAGTGCGTGTCTGGTCAAAACACTAACACCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101899 representing NP_041327
  Red=Cloning sites Green=Tags
  
  MADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDDENENDSDTGEDLVDFIVNDNDYLTQAETETAHAL
  FTAQEAKQHRDAVQVLKRKYLVSPLSDISGCVDNNISPRLKAICIEKQSRAAKRRLFESEDSGYGNTEVE
  TQQMLQVEGRHETETPCSQYSGGSGGGCSQYSSGSGGEGVSERHTICQTPLTNILNVLKTSNAKAAMLAK
  FKELYGVSFSELVRPFKSNKSTCCDWCIAAFGLTPSIADSIKTLLQQYCLYLHIQSLACSWGMVVLLLVR
  YKCGKNRETIEKLLSKLLCVSPMCMMIEPPKLRSTAAALYWYKTGISNISEVYGDTPEWIQRQTVLQHSF
  NDCTFELSQMVQWAYDNDIVDDSEIAYKYAQLADTNSNASAFLKSNSQAKIVKDCATMCRHYKRAEKKQM
  SMSQWIKYRCDRVDDGGDWKQIVMFLRYQGVEFMSFLTALKRFLQGIPKKNCILLYGAANTGKSLFGMSL
  MKFLQGSVICFVNSKSHFWLQPLADAKIGMLDDATVPCWNYIDDNLRNALDGNLVSMDVKHRPLVQLKCP
  PLLITSNINAGTDSRWPYLHNRLVVFTFPNEFPFDENGNPVYELNDKNWKSFFSRTWSRLSLHEDEDKEN
  DGDSLPTFKCVSGQNTNTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001526
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001526.2, NP_041327
• RefSeq ORF: 1947
• Locus ID: 1489075
• MW: 72.9 kDa