E4 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101900

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV16gp5, partial [Human papillomavirus type 16], codon optimized for human cell expression, NP_041329

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101900 represents NCBI reference of NP_041329 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  TATTACGTGTTGCATCTCTGTCTGGCTGCTACGAAGTACCCTCTGCTGAAACTGCTTGGGAGCACCTGGC
  CAACAACTCCACCCCGCCCTATCCCTAAGCCTTCTCCATGGGCACCCAAAAAACACAGGAGGCTGAGTAG
  TGACCAGGACCAGTCCCAGACACCAGAAACCCCCGCAACACCACTTTCATGCTGCACCGAGACTCAGTGG
  ACAGTACTCCAGAGCTCCCTGCATCTCACGGCCCATACAAAGGATGGACTTACCGTCATCGTCACACTGC
  ACCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101900 representing NP_041329
  Red=Cloning sites Green=Tags
  
  YYVLHLCLAATKYPLLKLLGSTWPTTPPRPIPKPSPWAPKKHRRLSSDQDQSQTPETPATPLSCCTETQW
  TVLQSSLHLTAHTKDGLTVIVTLHP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001526
• ORF Size: 285 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001526.2, NP_041329
• RefSeq ORF: 285
• Locus ID: 1489076
• MW: 10.6 kDa