E2 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101904

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041328

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101904 represents NCBI reference of NP_041328 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACACTCTGCCAAAGACTCAACGTTTGTCAGGACAAAATCCTGACACACTACGAGAACGATTCAA
  CAGACCTCAGAGACCATATTGACTACTGGAAGCATATGCGCCTGGAATGTGCCATATACTATAAAGCTAG
  AGAAATGGGGTTTAAGCACATCAACCATCAAGTCGTGCCTACTCTGGCTGTATCTAAAAATAAAGCGCTG
  CAGGCAATTGAGCTTCAGCTCACCCTCGAAACTATATATAACTCACAATACTCTAATGAAAAATGGACAC
  TTCAGGACGTGTCTTTGGAAGTTTACCTCACCGCTCCAACAGGGTGTATCAAGAAACACGGCTACACAGT
  CGAGGTTCAATTTGATGGCGATATCTGTAACACCATGCACTACACGAACTGGACCCACATCTATATTTGT
  GAGGAAGCCTCTGTTACCGTCGTGGAGGGCCAGGTGGATTACTACGGCCTTTATTACGTGCATGAGGGCA
  TAAGAACGTATTTCGTCCAGTTTAAGGATGACGCTGAGAAGTATAGTAAAAACAAGGTGTGGGAAGTGCA
  TGCCGGCGGTCAGGTCATCCTGTGCCCTACTAGTGTGTTCAGCTCCAACGAAGTCAGCAGTCCCGAGATA
  ATCCGACAACACCTTGCAAACCACCCCGCTGCAACACATACTAAAGCCGTGGCGCTCGGAACTGAAGAGA
  CACAAACTACCATCCAGAGACCCCGCAGCGAGCCTGATACGGGGAACCCTTGCCACACAACCAAATTGCT
  CCACAGAGACAGTGTGGACTCAGCGCCAATTCTGACGGCATTTAATTCAAGCCATAAGGGCCGCATCAAT
  TGTAACTCTAATACTACCCCCATTGTGCACCTCAAGGGAGATGCCAATACGTTGAAGTGTCTTCGCTACC
  GGTTTAAAAAACACTGCACTCTGTACACAGCCGTGTCCTCAACTTGGCATTGGACGGGCCACAACGTCAA
  ACATAAAAGTGCTATTGTCACCCTGACCTATGATTCCGAGTGGCAGCGCGACCAGTTTCTGTCACAGGTC
  AAAATTCCAAAGACGATTACTGTCTCTACTGGCTTCATGAGTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101904 representing NP_041328
  Red=Cloning sites Green=Tags
  
  METLCQRLNVCQDKILTHYENDSTDLRDHIDYWKHMRLECAIYYKAREMGFKHINHQVVPTLAVSKNKAL
  QAIELQLTLETIYNSQYSNEKWTLQDVSLEVYLTAPTGCIKKHGYTVEVQFDGDICNTMHYTNWTHIYIC
  EEASVTVVEGQVDYYGLYYVHEGIRTYFVQFKDDAEKYSKNKVWEVHAGGQVILCPTSVFSSNEVSSPEI
  IRQHLANHPAATHTKAVALGTEETQTTIQRPRSEPDTGNPCHTTKLLHRDSVDSAPILTAFNSSHKGRIN
  CNSNTTPIVHLKGDANTLKCLRYRFKKHCTLYTAVSSTWHWTGHNVKHKSAIVTLTYDSEWQRDQFLSQV
  KIPKTITVSTGFMSI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001526
• ORF Size: 1095 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001526.2, NP_041328
• RefSeq ORF: 1095
• Locus ID: 1489080
• MW: 41.8 kDa