E5 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101901

Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041330

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101901 represents NCBI reference of NP_041330 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAACCTTGACACCGCATCAACTACATTGTTGGCATGTTTCCTCCTGTGCTTCTGCGTGCTGCTCT
  GCGTCTGTTTGCTGATTCGCCCTTTGCTCCTGTCCGTATCCACCTACACCAGTCTGATCATCCTGGTCCT
  GCTTCTGTGGATTACAGCAGCTTCCGCCTTCCGCTGTTTTATCGTATACATAATATTCGTGTACATCCCT
  TTGTTTCTTATTCATACACACGCCAGGTTCCTGATCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101901 representing NP_041330
  Red=Cloning sites Green=Tags
  
  MTNLDTASTTLLACFLLCFCVLLCVCLLIRPLLLSVSTYTSLIILVLLLWITAASAFRCFIVYIIFVYIP
  LFLIHTHARFLIT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001526
• ORF Size: 249 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001526.2, NP_041330
• RefSeq ORF: 249
• Locus ID: 1489077
• MW: 9.4 kDa