E7 (NC_004500) Virus Tagged ORF Clone

CAT#: VC102029

Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 92], codon optimized for human cell expression, NP_775306

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102029 represents NCBI reference of NP_775306 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGGCAAGCAGGCAACAATTCCAGATATAGTACTTGACCTCCAGGACTTGGTGCAACCTATCGATC
  TGCACTGCGACGAGGATCTCTCTGAGAATCAAGAAGAGGAGCCTGCACCCCAGCGCATCGATTACAAGAT
  CGTATCATCCTGCGGGGGCTGCGGCATTAAACTGCGGATCTTCGCTAGCTGCACACAGTTCGGTATTAGA
  ACTCTCCAGGACCTGCTCCTCGAGGAAATTGCCCTGCTGTGTCCCGACTGCAAGAACGGGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102029 representing NP_775306
  Red=Cloning sites Green=Tags
  
  MIGKQATIPDIVLDLQDLVQPIDLHCDEDLSENQEEEPAPQRIDYKIVSSCGGCGIKLRIFASCTQFGIR
  TLQDLLLEEIALLCPDCKNGR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004500
• ORF Size: 273 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004500.1, NP_775306
• RefSeq ORF: 273
• Locus ID: 956298
• MW: 10.1 kDa