E1 (NC_004500) Virus Tagged ORF Clone

CAT#: VC102030

Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 92], codon optimized for human cell expression, NP_775307

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102030 represents NCBI reference of NP_775307 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACAAAGGGATTGATCCCAAGGAGGGTTGCTCAACTTGGTGTTTGATCGAAGCTGACTGCTCCG
  ATGTTGACGGCGATTTCGAAAAGCTGTTCGATAAGGACACTGACTCCGATATTTCTGATCTGCTCGACGA
  CGGAGATTTGGGTGATGCTGAACTCGGAAACCCTCAGGAACTGCTCTGTCTTCAAGAGAGAGAAGAATCA
  GATCTCCAACTCCAGCAGCTTAAGAGGAAGTACTTTTCTCCTAAAGCAGTACTCCAGCTTTCCCCACAGT
  TGGAGTCCATCACGATATCACCACAGCGCAAGTCAAAAAGACGACTCTTTGAAGAACAGGACAGCGGCCT
  CGAGCTCTCTTTGACACATGAAGCTGAGGACAGCGTTGCAGAGGTCGAGGTACCCGGTAGTAAAGACGAC
  GTGCCAGAAACTGTGTCAGCCACGGCAGAGACTAAGGGGAGTCAGAACAAGGAACACTACAAGCAGCTCC
  TTCAATGCAGTAACGCAAGAGCTACACTGCTCTCAAAGTTCAAGGCAGCCTTCGGAGTCAGCTTCACCGA
  GTTGACACGACGCTATAAGAGCGACAACACCTGCTGCAGAGATTGGGCGATCATAGTGTATGGCCTCCAA
  GACGAGATTATTGAGGGATCTAAGCATCTCTTTCAGCAACACTGCGAGTATATTTGGCTTCACGTACTGT
  CTCCCATCAGCCTGTATCTCCTCTGCTTCAAGACAGGTAAATCTCGCAACACAGTGAAAAATTTGTTGAT
  GTCAATTTTGAATGTTGGCGACGCCCAGCTCATCGCCGACCCGCCTCAGATCAGATCAGTGGTCGCCGCG
  CTGTTCTGGTACAAGGAATCTATGAACAAGAACGTGTATACCCACGGGGAGTATCCCGAATGGATCGCCA
  ACCAGACCCTGCTGTCCCATCAAGAGTACGAAACCCAACAATTTGACCTTTCACGAATGATTCAGTGGGC
  ATACGACAATGAGTACACTGAGGATTCCGACATTGCTTATCATTACGCCAAATTGGCAGACGAGGACAGT
  AATGCCCGCGCCTTCTTGGCCCATAACAGCCAGGCCAAATTCGTCCGAGAATGCGGACAAATGGTGAGAC
  ATTACAAACGCGGCGAAATGAAGAATATGAGTATGAGCGCTTGGATATACACGCGGCTGAAATCAATAGA
  AGGACCAGGGCACTGGTCTGATATTGTGAAGTTTATCCGCTTTCAGCAGATTAATTTTATTATGTTTCTG
  GATGTCTTCAAACAGTTCTTGGCCTCCGTTCCCAAGAGGAATTGCCTCCTTATCTACGGGGCACCGGATT
  GTGGGAAATCCATGTTTTGCATGTCCCTGATTAAAGCTCTGAAGGGCAAGGTGATTTCATTCGTTAACGC
  CCGGTCACAGTTTTGGTTGTCCCCCCTGGTTGAAAGCAAAATCGCATTGCTGGATGACGCGACAGAATGC
  TGTTGGAATTATATAGATAACTACCTGCGGAACGGAATTGATGGAAATATGGTTAGCGTCGATTGTAAGC
  ACAAAAACCCCGTGCAAATTAGATTTCCCCCCCTGCTGATAACTTCAAACAATAATATAATGTCAGATCC
  AAAATACAAGTATCTGCACAGCCGGATCAAGGCATTCGAATTCGTGAATAAGTTTCCTTTTAAGGATGAC
  GGCAGCCCCCTGTTTGAGCTGACCGACCAGTCCTGGAAGAGCTTTTTCCAGCGGCTTTGGCGGCAGTTGG
  ATCTTAGCGATCAGGAGGACGAAGGCGAGGATGGCGGCAGTCAGCGCCCCTTTCAGTGTACCGCCAGACA
  GGTCAATGACAACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102030 representing NP_775307
  Red=Cloning sites Green=Tags
  
  MADKGIDPKEGCSTWCLIEADCSDVDGDFEKLFDKDTDSDISDLLDDGDLGDAELGNPQELLCLQEREES
  DLQLQQLKRKYFSPKAVLQLSPQLESITISPQRKSKRRLFEEQDSGLELSLTHEAEDSVAEVEVPGSKDD
  VPETVSATAETKGSQNKEHYKQLLQCSNARATLLSKFKAAFGVSFTELTRRYKSDNTCCRDWAIIVYGLQ
  DEIIEGSKHLFQQHCEYIWLHVLSPISLYLLCFKTGKSRNTVKNLLMSILNVGDAQLIADPPQIRSVVAA
  LFWYKESMNKNVYTHGEYPEWIANQTLLSHQEYETQQFDLSRMIQWAYDNEYTEDSDIAYHYAKLADEDS
  NARAFLAHNSQAKFVRECGQMVRHYKRGEMKNMSMSAWIYTRLKSIEGPGHWSDIVKFIRFQQINFIMFL
  DVFKQFLASVPKRNCLLIYGAPDCGKSMFCMSLIKALKGKVISFVNARSQFWLSPLVESKIALLDDATEC
  CWNYIDNYLRNGIDGNMVSVDCKHKNPVQIRFPPLLITSNNNIMSDPKYKYLHSRIKAFEFVNKFPFKDD
  GSPLFELTDQSWKSFFQRLWRQLDLSDQEDEGEDGGSQRPFQCTARQVNDNL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004500
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004500.1, NP_775307
• RefSeq ORF: 1836
• Locus ID: 956300
• MW: 70.3 kDa