L2 (NC_004500) Virus Tagged ORF Clone

CAT#: VC102033

Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 92], codon optimized for human cell expression, NP_775310

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102033 represents NCBI reference of NP_775310 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGAGCCCGGCGGACCAAGAGGGATAGCGTCACAAACATTTACCGCACCTGCAAGGCTGCGGGTA
  CCTGCCCCCCCGATGTGGTGAACAAAGTTGAACAGACTACCGTTGCCGACCAGATCCTGAAGTACGGGTC
  TACCGGAGTGTTCTTTGGCGGGCTGGGCATCGGTACGGGACGCGGCACGGGTGGTTCTACCGGTTACGGC
  CCACTGGGCGAAGGAACCAGTGTGCGGGTAGGCAACACTCCTACGGTGATCAGGCCTGCGCTGGTACCGG
  AGGCTATTGGACCATCTGAGCTTATTCCAATCGATTCTGTCAATCCCATCGACCCGAGTGCCTCAAGCAT
  TATCCCCCTCACTGAGTCCACCGGTCCGGATTTGCTGCCGGGTGAGATCGAGACCATCGCCGAGGTCCAT
  CCTGCCCCCGACATCCCTACTGTGGATACCCCAGTGGTCACAGGCGGGCGAAACAGTAATGCTGTACTTG
  AGGTTGCTGACCCTTCCCCACCCACTCGGAACCGCGTAAGCCGGACTCAGTATAATAACCCTGCCTTCCA
  GATCATAAGCGAGACTACACCCTCTGCCGGAGAGACGTCACTCTCAGATCAGATAGTGGTGCAATCCTTT
  GACGGTGGTCAATATATTGGGGGAAATCCGCCCCCGAGATCTGTAGTGGAAATTGAGCTCCAGGAAATAC
  CCTCCCAGTATAGCTTCGAGATAGAGGAACCTACCCCTCCCAGACAGACCAGCACCCCCGTACGGCAGGC
  TCAGCAGATGGCGAGTGCCCTGAGACGGGCTCTGTATAACCGCAGATTTACCCAGCAGGTCCAGGTCGAG
  GACCCTATGTTTTATTCACGCCCTAGTCGGCTTGTTAGGTTCCAATTCGATAATCCGGTATTTGAGGAAG
  AAGTAACGCAGGTCTTTGAACGCGACCTCGAGACTATTGAAGAGCCCCCCGACCGGCAATTCCTCGACGT
  GCAGAAGCTGGGGAGACCGACCTACGCAGAAACTCCAGCTGGCTATATCAGGGTTTCTAGGCTCGGAAAA
  CGCGCGACCATCAGAACACGATCTGGCACAACTATCGGCGGTCAGGTGCATTTCTTCAGGGACATCAGCT
  CTATAGATACACAGCCTTCAATCGAGCTGCAGGTGCTGGGTGAGCATAGCGGCGACGCAACAATCGTCCA
  GGGGCCTGTGGAATCCACCTTCGTGAATATTGACCTGGAAGAGCTCCCAAATCTCGAGGAGAATGTACAT
  TTGGAAAGCGACGACATCCTGATAGACGAGGCTATTGAGGATTTCTCTGGTGCTCAGCTGGTCTTCGGGA
  ACAGCCGACGCAGCAACACTGTCACCCTCCCAAGGTTTGAGACCGTTCGAGAAACCTCCTTGTATACCGT
  GGATCTCGACGGGTTCCATGTGAGTTATCCAGAGTCCAGAGCCTACCCTGAGGTTATTCCTACCGAGCCT
  GACAACACACCGACCGTGATCATTCACACGGAGGACTTTAGCGGGGATTATTACCTGCACCCATCCTTGA
  AATGGAAAAAAAGGAAAAGAGCATACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102033 representing NP_775310
  Red=Cloning sites Green=Tags
  
  MARARRTKRDSVTNIYRTCKAAGTCPPDVVNKVEQTTVADQILKYGSTGVFFGGLGIGTGRGTGGSTGYG
  PLGEGTSVRVGNTPTVIRPALVPEAIGPSELIPIDSVNPIDPSASSIIPLTESTGPDLLPGEIETIAEVH
  PAPDIPTVDTPVVTGGRNSNAVLEVADPSPPTRNRVSRTQYNNPAFQIISETTPSAGETSLSDQIVVQSF
  DGGQYIGGNPPPRSVVEIELQEIPSQYSFEIEEPTPPRQTSTPVRQAQQMASALRRALYNRRFTQQVQVE
  DPMFYSRPSRLVRFQFDNPVFEEEVTQVFERDLETIEEPPDRQFLDVQKLGRPTYAETPAGYIRVSRLGK
  RATIRTRSGTTIGGQVHFFRDISSIDTQPSIELQVLGEHSGDATIVQGPVESTFVNIDLEELPNLEENVH
  LESDDILIDEAIEDFSGAQLVFGNSRRSNTVTLPRFETVRETSLYTVDLDGFHVSYPESRAYPEVIPTEP
  DNTPTVIIHTEDFSGDYYLHPSLKWKKRKRAYL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004500
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004500.1, NP_775310
• RefSeq ORF: 1569
• Locus ID: 956301
• MW: 57.6 kDa