E2 (NC_004500) Virus Tagged ORF Clone

CAT#: VC102031

Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 92], codon optimized for human cell expression, NP_775308

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102031 represents NCBI reference of NP_775308 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCTCTGTCCGACAGGTTCAATGCTCTTCAGGACAAGCTGATGACTATCTATGAGCGCGCCAGTG
  AGTCCCTCAAAGACCAAATCGAACACTGGAACCTTTTGAGGCAGGAGCAGGTTCTGTTCCACTACGCCCG
  ACAACGGGGTGTGCTCCGCCTTGGATATCAACCCGTGCCCGCACTGACTATCTCTGAGGCCAAGGCTAAG
  GAGGCCATTGCCATGGTCCTGCACCTCGAGGCATTGCAGAGATCTCCCTATAAAAACGAGAAGTGGACCC
  TTGTGAATACTAGTGTCGAAACTTTCCGCACCCCGCCAGAGAACTGCTTCAAAAAAGGGCCTAAGACCAT
  CGAAATCGTGTACGACGGGAATCCCGAAAACACAATGCTTTATACAATCTGGACCCACATTTATTTTGAG
  GACGATGAGGGCAACTGGCAGAAGACCGAGGGACACCTTGACTACCACGGTGCATACTTTATGGACGGGC
  TCAACAAACAGTACTACATCAGGTTTGCGCAGGATGCACGAAGATTTAGCGAGACCGGCGAATGGGAAGT
  GAAGTTCAATAACGAGATCCTGTTCGCACCAGTGACCTCTAGCACCAACTCTGAAGAAGAAAGGGACCGC
  CCAGCACCAGCAACCGACCCAGGTAGCCTGTCTCAAACCAGCGGTGGCCAGTCACCCGTCCCTACACAGA
  GGAAACAACCATCTAAGGGTAGATACGGCAGAAAGGATTCTGGAGCCACAACAGCTTCAAGGGGTATTCA
  GAGGCGACCTAAGGCGTCTCCCAGGAGGAGTAGAAGCCGATCAGGCAGTCGCTCCGGCAGTCAGGGCGAC
  GCTCGAACCCTGTTGACAGTAAGAAGAGGAGAGAGAGAACGGGGTCAGGGAAGAGGCCAGGGCAGCAGAG
  GCCGGGGGCGGTCAGGAGACCGCTCAAGATCCGGATCCCGCAGTAGATCAGGCAGCCGAAGGAGATCAAG
  ATCAAGGGGCCGCTCACGAAATGGACGCGAGAGGGGCAGGGCTGCATCTCGCGGGAGGCGCGGCTATTCA
  AACCGGAGGTCTCGATCCAAAAGCGTGGGCACTAGCGGCATCCCCCCCGAACAAGTAGGGAGCTCCTTGC
  AGGGGGTCGGCAGGCAGCATAGCGGCAGGCTGGCTAGACTTCTCGATGACGCTCGGGATCCCCCAGTTAT
  ACTGTTGAAGGGCCAGGCAAATACTCTGAAATGTTACAGATACAGAGCAAAGGAAAAGTATAAGGGGTAT
  TATGATTGTTTCTCAACCACATGGTCCTGGGTGTCTGCAGGGTCCAATGACCGGATCGGGCGGTCCAGGA
  TGATCATTTCCTTCACGAGCAAGTCCCAGAGGCAGATGTTTCTTAGCATTATGAAACTGCCCAAGGGCGT
  TGACTGGAGCCTGGGATGCTTCGATTCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102031 representing NP_775308
  Red=Cloning sites Green=Tags
  
  MEALSDRFNALQDKLMTIYERASESLKDQIEHWNLLRQEQVLFHYARQRGVLRLGYQPVPALTISEAKAK
  EAIAMVLHLEALQRSPYKNEKWTLVNTSVETFRTPPENCFKKGPKTIEIVYDGNPENTMLYTIWTHIYFE
  DDEGNWQKTEGHLDYHGAYFMDGLNKQYYIRFAQDARRFSETGEWEVKFNNEILFAPVTSSTNSEEERDR
  PAPATDPGSLSQTSGGQSPVPTQRKQPSKGRYGRKDSGATTASRGIQRRPKASPRRSRSRSGSRSGSQGD
  ARTLLTVRRGERERGQGRGQGSRGRGRSGDRSRSGSRSRSGSRRRSRSRGRSRNGRERGRAASRGRRGYS
  NRRSRSKSVGTSGIPPEQVGSSLQGVGRQHSGRLARLLDDARDPPVILLKGQANTLKCYRYRAKEKYKGY
  YDCFSTTWSWVSAGSNDRIGRSRMIISFTSKSQRQMFLSIMKLPKGVDWSLGCFDSI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004500
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004500.1, NP_775308
• RefSeq ORF: 1431
• Locus ID: 956299
• MW: 54.1 kDa