Polymerase (NC_003977) Virus Tagged ORF Clone

CAT#: VC102192

Myc-DDK-tagged ORF clone of viral ORF for Polymerase [Hepatitis B virus], codon optimized for human cell expression, NP_647604

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polymerase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102192 represents NCBI reference of NP_647604 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACTGAGTTACCAGCATTTTAGAAAGCTGCTGCTTCTGGACGACGAGGCAGGCCCTCTGGAGGAAG
  AGCTGCCGCGGCTGGCCGACGAGGGATTGAATCGCCGGGTGGCCGAGGACCTGAATCTCGGAAACCTCAA
  TGTTTCCATCCCCTGGACCCACAAGGTGGGCAACTTTACAGGCCTGTACTCCTCCACGGTCCCCGTTTTT
  AACCCCGACTGGAAAACACCTTCCTTTCCCCACATACATCTTCAGGAGGACATTATTAACAGGTGCCAGC
  AGTATGTCGGCCCTCTCACTGTTAACGAGAAAAGGCGGCTGAAGCTGATCATGCCCGCTAGATTCTACCC
  CAATCTGACCAAATACCTGCCCCTGGACAAGGGTATCAAGCCGTATTATCCCGAGTATGCCGTTAATCAT
  TACTTTAAAACACGGCACTATTTGCATACCCTGTGGAAAGCCGGTATTCTGTATAAAAGAGAGACCACCA
  GGTCAGCCTCATTTTGCGGAAGCCCATACTCTTGGGAACAGGAGCTGCAGCACGGCCGACTGGTCTTTCA
  AACCTCTACCAGGCATGGCGATGAGTCCTTCTGCTCTCAAAGCTCAGGAATTCTGTCACGCTCACCAGTA
  GGCCCCTGTGTGAGATCTCAGCTGAAACAGTCTCGCCTTGGCCTTCAACCGCAGCAGGGAAGTCTGGCGC
  GGGGTAAGTCAGGCCGATCCGGCTCCATTTGGTCCAGAGTGCATCCTACAACACGGCGCCCTTTCGGGGT
  CGAACCTTCAGGATCAGGACACATAGATAACACAGCCTCCTCAACATCCTCATGCCTCCACCAGTCTGCC
  GTCCGCAAAACAGCCTATAGTCATCTCTCTACGTCCAAGAGGCAATCCAGCTCAGGACATGCCGTTGAGC
  TGCACAACATTCCCCCCTCTAGCGCTAGATCTCAGAGCGAAGGGCCTATCTTTTCATGTTGGTGGCTGCA
  GTTCAGGAACAGCAAGCCGTGTAGTGATTATTGCCTGACTCACATCGTGAACCTTCTGGAGGATTGGGGG
  CCTTGCACTGAGCACGGTGAACATAATATTCGGATTCCCCGAACCCCTGCTCGGGTGACAGGAGGCGTGT
  TCCTTGTGGATAAGAATCCCCACAACACAACCGAGTCCAGACTCGTCGTGGACTTCAGCCAGTTCAGCCG
  GGGATCTACACACGTTTCCTGGCCCAAATTCGCAGTCCCTAACTTGCAAAGCCTGACGAATTTGCTGTCC
  TCAAATCTCTCCTGGCTCAGCCTGGACGTCAGCGCTGCTTTTTATCACATCCCCCTCCACCCTGCTGCGA
  TGCCACACCTCTTGGTGGGGAGCTCTGGACTTCCCCGCTATGTCGCCAGACTGAGTTCAACTTCTCGCAA
  TATCAACTATCAGCATGGTACAATGCAAAATCTCCACGATTCTTGCTCTAGGAACCTGTACGTGAGCCTG
  CTCCTGTTGTACAAAACCTTCGGTCGCAAGTTGCATCTGTATTCCCATCCCATCATCTTGGGTTTTAGGA
  AAATCCCGATGGGAGTGGGACTCTCTCCCTTCCTTCTCGCCCAGTTCACCTCCGCCATTTGTAGTGTCGT
  CAGGCGAGCGTTTCCCCATTGTCTGGCTTTCTCTTATATGGATGACGTTGTACTGGGTGCTAAGTCAGTC
  CAACATCTGGAGTCTCTCTTCACAAGTATCACCAACTTTTTGCTGTCCTTGGGGATCCACCTCAACCCCA
  ACAAGACTAAGAGATGGGGGTACTCACTTAATTTCATGGGATATGTAATCGGAAGTTGGGGAACGCTTCC
  GCAGGAGCACATCGTTCAGAAACTCAAGCAGTGTTTTCGCAAGCTCCCCGTTAACCGCCCTATAGACTGG
  AAGGTTTGTCAGCGCATTGTCGGGCTTTTGGGATTCGCAGCTCCTTTCACCCAGTGCGGCTACCCTGCTC
  TTATGCCTCTGTACGCTTGTATCCAGAGCAAGCAGGCATTCACTTTCTCACCGACCTACAAAGCATTTCT
  GTGCAAGCAATACCTGAACCTCTACCCCGTGGCAAGGCAGCGCAGCGGGCTTTGCCAGGTCTTTGCCGAC
  GCCACCCCTACCGGCTGGGGTTTGGCTATTGGACACCGGAGAATGAGAGGCACATTCGTCGCTCCGCTGC
  CCATCCATACAGCCGAACTGCTCGCCGCCTGTTTTGCTCGGTCCAGATCAGGCGCCAAACTGATCGGAAC
  AGACAACTCTGTAGTGCTGTCACGGAAATATACGTCCTTCCCCTGGCTGCTCGGGTGCGCCGCAAACTGG
  ATCCTGAGAGGTACCAGCTTCGTTTATGTACCCTCTGCGCTGAACCCCGCCGACGACCCAAGCAGAGGAA
  GACTGGGACTTTACCGCCCCTTGCTCCACCTGCCATTCCGACCCACAACAGGTCGGACTTCCCTTTACGC
  AGTCTCCCCTAGCGTGCCCTCACACCTGCCGGACCGGGTGCATTTCGCTAGCCCACTGCACGTTGCGTGG
  AGACCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102192 representing NP_647604
  Red=Cloning sites Green=Tags
  
  MPLSYQHFRKLLLLDDEAGPLEEELPRLADEGLNRRVAEDLNLGNLNVSIPWTHKVGNFTGLYSSTVPVF
  NPDWKTPSFPHIHLQEDIINRCQQYVGPLTVNEKRRLKLIMPARFYPNLTKYLPLDKGIKPYYPEYAVNH
  YFKTRHYLHTLWKAGILYKRETTRSASFCGSPYSWEQELQHGRLVFQTSTRHGDESFCSQSSGILSRSPV
  GPCVRSQLKQSRLGLQPQQGSLARGKSGRSGSIWSRVHPTTRRPFGVEPSGSGHIDNTASSTSSCLHQSA
  VRKTAYSHLSTSKRQSSSGHAVELHNIPPSSARSQSEGPIFSCWWLQFRNSKPCSDYCLTHIVNLLEDWG
  PCTEHGEHNIRIPRTPARVTGGVFLVDKNPHNTTESRLVVDFSQFSRGSTHVSWPKFAVPNLQSLTNLLS
  SNLSWLSLDVSAAFYHIPLHPAAMPHLLVGSSGLPRYVARLSSTSRNINYQHGTMQNLHDSCSRNLYVSL
  LLLYKTFGRKLHLYSHPIILGFRKIPMGVGLSPFLLAQFTSAICSVVRRAFPHCLAFSYMDDVVLGAKSV
  QHLESLFTSITNFLLSLGIHLNPNKTKRWGYSLNFMGYVIGSWGTLPQEHIVQKLKQCFRKLPVNRPIDW
  KVCQRIVGLLGFAAPFTQCGYPALMPLYACIQSKQAFTFSPTYKAFLCKQYLNLYPVARQRSGLCQVFAD
  ATPTGWGLAIGHRRMRGTFVAPLPIHTAELLAACFARSRSGAKLIGTDNSVVLSRKYTSFPWLLGCAANW
  ILRGTSFVYVPSALNPADDPSRGRLGLYRPLLHLPFRPTTGRTSLYAVSPSVPSHLPDRVHFASPLHVAW
  RPP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003977
• ORF Size: 2529 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003977.1, NP_647604
• RefSeq ORF: 2529
• Locus ID: 944565
• MW: 94.6 kDa