middle S protein (NC_003977) Virus Tagged ORF Clone

CAT#: VC102197

Myc-DDK-tagged ORF clone of viral ORF for middle S protein [Hepatitis B virus], codon optimized for human cell expression, YP_355334

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: middle S protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102197 represents NCBI reference of YP_355334 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGGAATTCAACAACTTTTCACCAGGCCCTGCTGGACCCACGGGTGAGAGGTCTCTATTTTCCGG
  CCGGTGGCAGCTCCTCAGGAACTGTTAATCCCGTTCCTACTACGGCCAGTCCTATCTCCAGCATCTTCTC
  TAGGACCGGGGATCCTGCACCGAACATGGAGTCCACGACATCCGGTTTTCTGGGGCCTCTGTTGGTGCTT
  CAGGCAGGCTTTTTCCTTCTTACGCGAATTCTCACAATCCCGCAGTCATTGGATAGCTGGTGGACCTCCC
  TCAACTTCCTCGGGGGCGCCCCCACATGCCCTGGACAGAATTCTCAGAGCCCTACGTCAAATCACAGTCC
  CACGTCTTGCCCACCCACGTGTCCAGGCTACAGATGGATGTGTCTCCGCCGCTTTATAATTTTCCTTTTT
  ATCCTCCTCCTCTGCCTTATTTTCCTGCTTGTGCTGCTCGACTATCAGGGAATGCTGCCCGTGTGCCCAC
  TTCTCCCTGGAACTTCAACCACCTCCACCGGACCCTGCCGAACCTGTACTATCCCTGCTCAGGGGACTTC
  TATGTTCCCTAGTTGCTGCTGCACAAAACCTTCCGACGGCAACTGTACCTGTATTCCTATCCCCAGTAGT
  TGGGCCTTCGCCAGATTTCTGTGGGAATGGGCGTCTGTGCGCTTTTCCTGGCTGTCCCTGTTGGTCCCTT
  TCGTCCAGTGGTTCGTAGGGTTGTCTCCAACAGTTTGGCTTAGTGCAATCTGGATGATGTGGTATTGGGG
  TCCAAGCTTGTATAACATACTCTCTCCCTTCTTGCCACTGCTGCCTATTTTTTTCTGCTTGTGGGTGTAC
  ATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102197 representing YP_355334
  Red=Cloning sites Green=Tags
  
  MQWNSTTFHQALLDPRVRGLYFPAGGSSSGTVNPVPTTASPISSIFSRTGDPAPNMESTTSGFLGPLLVL
  QAGFFLLTRILTIPQSLDSWWTSLNFLGGAPTCPGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLF
  ILLLCLIFLLVLLDYQGMLPVCPLLPGTSTTSTGPCRTCTIPAQGTSMFPSCCCTKPSDGNCTCIPIPSS
  WAFARFLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYNILSPFLPLLPIFFCLWVY
  I
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003977
• ORF Size: 843 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003977.1, YP_355334
• RefSeq ORF: 843
• Locus ID: 944569
• MW: 31.2 kDa