Core and e antigen (NC_003977) Virus Tagged ORF Clone

CAT#: VC102195

Myc-DDK-tagged ORF clone of viral ORF for Core and e antigen [Hepatitis B virus], codon optimized for human cell expression, NP_647607

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Core and e antigen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102195 represents NCBI reference of NP_647607 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATAGATCCGTACAAAGAGTTCGGTGCCAGTGTTGAACTGCTGAGCTTCTTGCCGTCAGACTTTT
  TTCCATCTATTCGCGACCTTTTGGACACCGCAAGCGCCCTTTACCGCGAGGCCCTGGAGAGCCCTGAGCA
  CTGCAGCCCTCACCACACTGCACTGAGACAAGCCATCCTGTGTTGGGGCGAACTGATGAACCTGGCAACC
  TGGGTCGGATCTAATTTGGAAGACCCTGCTAGTCGGGAGCTGGTGGTTTCTTATGTCAACGTAAATATGG
  GTCTGAAGATCCGACAGCTTCTGTGGTTCCACATATCCTGTCTGACATTCGGGCGCGAAACTGTGCTCGA
  GTACCTTGTGAGCTTTGGAGTCTGGATCCGCACTCCCCCTGCTTATAGGCCGCCTAACGCCCCCATCCTG
  AGCACACTGCCCGAAACCACCGTGGTTAGGAGGAGAGGCAGAAGCCCCCGGAGACGGACACCTTCTCCTC
  GGAGAAGACGGAGCCAGTCTCCCAGAAGGCGCCGCAGCCAATCTAGGGAGAGTCAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102195 representing NP_647607
  Red=Cloning sites Green=Tags
  
  MDIDPYKEFGASVELLSFLPSDFFPSIRDLLDTASALYREALESPEHCSPHHTALRQAILCWGELMNLAT
  WVGSNLEDPASRELVVSYVNVNMGLKIRQLLWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPIL
  STLPETTVVRRRGRSPRRRTPSPRRRRSQSPRRRRSQSRESQC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003977
• ORF Size: 549 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003977.1, NP_647607
• RefSeq ORF: 549
• Locus ID: 944568
• MW: 21.1 kDa