large S protein (NC_003977) Virus Tagged ORF Clone

CAT#: VC102196

Myc-DDK-tagged ORF clone of viral ORF for large S protein [Hepatitis B virus], codon optimized for human cell expression, YP_355333

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: large S protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102196 represents NCBI reference of YP_355333 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCTGGAGCTCTAAACCCAGACAGGGCATGGGAACTAACCTGAGCGTGCCAAATCCACTTGGGT
  TCTTTCCCGACCACCAGCTGGACCCAGCTTTCGGCGCCAATAGCAACAACCCGGATTGGGACTTCAACCC
  AAACAAAGACCATTGGCCTGAAGCGAATCAGGTGGGAGCTGGAGCCTTCGGTCCAGGCTTCACACCCCCA
  CATGGCGGCCTGCTGGGGTGGAGCCCTCAAGCGCAAGGCATCTTGACCACACTTCCTGCAGCTCCTCCCC
  CCGCGTCCACTAATCGCCAGAGTGGCAGACAGCCAACACCAATATCACCGCCTCTTAGAGACTCTCACCC
  TCAGGCCATGCAGTGGAACAGCACCACTTTCCACCAGGCACTTCTGGACCCCAGGGTCAGAGGGCTGTAT
  TTCCCTGCCGGAGGCAGTTCCTCTGGCACTGTCAACCCGGTGCCGACCACGGCTTCTCCTATCTCCTCAA
  TTTTCTCAAGAACCGGCGACCCCGCACCCAACATGGAATCCACTACAAGCGGCTTCCTTGGCCCTCTTCT
  GGTGCTGCAGGCTGGTTTTTTCCTCCTGACGCGCATACTTACCATACCTCAGTCACTGGATTCTTGGTGG
  ACATCTCTGAATTTCCTGGGTGGGGCTCCCACATGTCCCGGCCAGAATAGCCAGTCTCCCACTTCCAACC
  ATTCCCCTACCAGCTGCCCCCCCACATGTCCAGGGTACCGGTGGATGTGCCTGAGGAGATTTATTATATT
  CCTCTTTATCCTCCTGCTTTGCCTGATTTTCCTCTTGGTTCTCCTTGATTATCAGGGGATGCTGCCCGTC
  TGTCCTCTGCTTCCTGGCACATCAACCACCTCAACCGGCCCATGCCGCACGTGTACCATACCCGCACAGG
  GGACATCTATGTTCCCTAGCTGCTGTTGCACTAAACCCAGCGACGGGAACTGTACGTGCATTCCCATCCC
  CTCCTCATGGGCCTTCGCCAGATTTCTGTGGGAGTGGGCCAGCGTAAGATTCTCTTGGTTGTCCCTCCTG
  GTCCCGTTCGTCCAGTGGTTCGTGGGCCTGAGCCCCACGGTGTGGTTGTCTGCGATCTGGATGATGTGGT
  ATTGGGGGCCTTCCCTGTACAACATTCTGTCTCCCTTCCTCCCTCTGCTCCCTATATTCTTCTGTCTCTG
  GGTGTATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102196 representing YP_355333
  Red=Cloning sites Green=Tags
  
  MGGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFGANSNNPDWDFNPNKDHWPEANQVGAGAFGPGFTPP
  HGGLLGWSPQAQGILTTLPAAPPPASTNRQSGRQPTPISPPLRDSHPQAMQWNSTTFHQALLDPRVRGLY
  FPAGGSSSGTVNPVPTTASPISSIFSRTGDPAPNMESTTSGFLGPLLVLQAGFFLLTRILTIPQSLDSWW
  TSLNFLGGAPTCPGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPV
  CPLLPGTSTTSTGPCRTCTIPAQGTSMFPSCCCTKPSDGNCTCIPIPSSWAFARFLWEWASVRFSWLSLL
  VPFVQWFVGLSPTVWLSAIWMMWYWGPSLYNILSPFLPLLPIFFCLWVYI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003977
• ORF Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003977.1, YP_355333
• RefSeq ORF: 1200
• Locus ID: 944569
• MW: 43.7 kDa