envelope protein (NC_009942) Virus Tagged ORF Clone

CAT#: VC102518

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, YP_001527880

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: envelope protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102518 represents NCBI reference of YP_001527880 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAATTGTCTTGGCATGAGCAATCGAGACTTTCTGGAAGGCGTCTCAGGCGCGACATGGGTGGACC
  TGGTGCTCGAGGGAGATTCATGCGTCACGATTATGTCTAAGGATAAACCTACCATAGATGTGAAAATGAT
  GAACATGGAAGCTGCAAACCTGGCAGAAGTCCGGTCTTACTGTTATTTGGCAACCGTCTCTGACCTGTCC
  ACAAAAGCAGCATGCCCAACTATGGGTGAGGCACACAATGACAAGAGAGCCGATCCCGCTTTTGTCTGCC
  GCCAAGGCGTTGTGGACCGCGGGTGGGGCAACGGATGTGGACTCTTCGGGAAAGGTAGCATAGACACTTG
  CGCCAAATTTGCCTGTAGTACGAAAGCCATTGGCAGGACAATACTGAAAGAAAACATCAAGTATGAAGTT
  GCTATCTTTGTACATGGGCCTACAACAGTCGAAAGCCACGGTAATTATTCAACTCAAGTGGGAGCTACGC
  AAGCCGGCAGGTTGAGCATTACTCCTGCCGCACCCTCCTACACTCTCAAACTGGGTGAATACGGGGAGGT
  GACGGTGGACTGTGAGCCTCGCTCCGGAATCGATACAAACGCATACTACGTTATGACTGTTGGGACCAAA
  ACGTTCCTGGTCCATCGAGAGTGGTTCATGGATCTGAACCTGCCCTGGTCTTCCGCCGGAAGTACGGTCT
  GGCGAAATAGAGAAACATTGATGGAGTTTGAAGAACCCCACGCTACCAAGCAAAGTGTTATAGCCCTTGG
  CTCACAGGAGGGCGCCCTCCACCAGGCACTGGCCGGGGCAATCCCCGTTGAGTTCTCTAGCAATACTGTG
  AAACTTACGAGTGGCCATCTGAAGTGCAGGGTTAAGATGGAAAAGCTGCAGCTGAAGGGCACAACATACG
  GAGTATGTTCTAAAGCATTTAAATTTCTTGGAACCCCTGCAGATACCGGACATGGCACAGTGGTTCTTGA
  ACTTCAGTATACTGGAACCGATGGCCCATGCAAGGTGCCTATAAGCAGCGTTGCCAGTTTGAATGATCTC
  ACTCCTGTGGGCCGACTCGTAACCGTTAACCCATTCGTTTCTGTGGCGACCGCCAACGCAAAGGTGTTGA
  TAGAGCTGGAGCCCCCGTTCGGGGACTCTTATATCGTGGTTGGCCGCGGGGAACAGCAGATAAACCATCA
  TTGGCACAAGTCCGGTAGTAGCATAGGCAAGGCATTCACTACTACCCTTAAAGGAGCTCAGAGACTCGCC
  GCTCTGGGAGATACCGCTTGGGACTTTGGCAGTGTTGGCGGCGTATTTACTAGCGTCGGTAAAGCTGTAC
  ATCAGGTTTTCGGCGGTGCCTTTAGATCCTTGTTTGGGGGAATGTCCTGGATTACCCAGGGTCTTCTGGG
  AGCCCTCCTTTTGTGGATGGGGATCAACGCCCGAGACCGCTCCATTGCCCTTACTTTCCTCGCCGTGGGA
  GGAGTGTTGTTGTTTTTGTCTGTGAACGTGCATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102518 representing YP_001527880
  Red=Cloning sites Green=Tags
  
  MFNCLGMSNRDFLEGVSGATWVDLVLEGDSCVTIMSKDKPTIDVKMMNMEAANLAEVRSYCYLATVSDLS
  TKAACPTMGEAHNDKRADPAFVCRQGVVDRGWGNGCGLFGKGSIDTCAKFACSTKAIGRTILKENIKYEV
  AIFVHGPTTVESHGNYSTQVGATQAGRLSITPAAPSYTLKLGEYGEVTVDCEPRSGIDTNAYYVMTVGTK
  TFLVHREWFMDLNLPWSSAGSTVWRNRETLMEFEEPHATKQSVIALGSQEGALHQALAGAIPVEFSSNTV
  KLTSGHLKCRVKMEKLQLKGTTYGVCSKAFKFLGTPADTGHGTVVLELQYTGTDGPCKVPISSVASLNDL
  TPVGRLVTVNPFVSVATANAKVLIELEPPFGDSYIVVGRGEQQINHHWHKSGSSIGKAFTTTLKGAQRLA
  ALGDTAWDFGSVGGVFTSVGKAVHQVFGGAFRSLFGGMSWITQGLLGALLLWMGINARDRSIALTFLAVG
  GVLLFLSVNVHA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009942
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009942.1, YP_001527880
• RefSeq ORF: 1506
• MW: 53.7 kDa