nonstructural protein 3 (NC_009942) Virus Tagged ORF Clone

CAT#: VC102522

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, YP_001527884

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102522 represents NCBI reference of YP_001527884 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGGTCCTCTGGGACACGCCCAGCCCTAAAGAATACAAGAAGGGCGATACAACCACAGGCGTGT
  ACAGGATCATGACTCGCGGCCTTCTGGGGTCCTACCAAGCTGGCGCCGGCGTTATGGTGGAGGGCGTGTT
  TCACACCCTCTGGCACACCACAAAGGGTGCCGCACTTATGTCCGGTGAAGGCCGACTGGACCCATATTGG
  GGGTCCGTGAAGGAGGATAGACTGTGCTACGGAGGGCCTTGGAAACTGCAGCACAAGTGGAACGGCCAGG
  ACGAAGTTCAGATGATAGTCGTGGAACCTGGAAAGAACGTGAAGAATGTACAGACCAAACCCGGCGTGTT
  TAAAACACCTGAAGGCGAGATTGGGGCTGTCACACTGGACTTTCCCACAGGTACTTCCGGAAGCCCCATC
  GTGGATAAGAATGGGGATGTGATCGGCCTGTATGGCAACGGCGTAATTATGCCCAATGGGAGTTACATCT
  CCGCTATAGTGCAGGGTGAGAGGATGGATGAGCCAATCCCTGCCGGCTTTGAACCCGAAATGTTGCGGAA
  AAAGCAAATCACAGTGCTGGACTTGCACCCAGGAGCCGGAAAGACCAGGCGCATACTGCCACAGATAATC
  AAGGAAGCTATCAACCGCAGACTCCGGACAGCCGTGTTGGCTCCCACCCGCGTTGTGGCGGCCGAGATGG
  CTGAAGCTTTGCGGGGGCTGCCCATTCGCTATCAAACATCAGCGGTGCCCCGCGAGCACAACGGTAATGA
  AATTGTGGATGTGATGTGTCACGCAACCCTGACTCATCGACTCATGAGCCCACACCGGGTGCCCAATTAT
  AACTTGTTCGTGATGGACGAAGCTCATTTTACTGACCCTGCTTCCATTGCTGCCCGGGGGTATATCTCAA
  CAAAGGTAGAACTCGGTGAGGCTGCAGCTATATTTATGACCGCCACTCCCCCCGGTACTAGCGACCCCTT
  TCCTGAGAGTAACTCACCAATATCCGACCTCCAAACCGAGATTCCCGACAGAGCTTGGAACTCTGGTTAC
  GAATGGATTACCGAGTACACGGGCAAGACTGTCTGGTTTGTTCCTTCCGTGAAAATGGGGAATGAGATCG
  CTCTCTGCTTGCAAAGAGCCGGAAAGAAAGTTGTCCAGCTCAATAGAAAAAGCTATGAGACCGAGTACCC
  CAAATGCAAGAATGACGACTGGGACTTTGTCATCACTACAGACATTTCAGAAATGGGTGCTAACTTTAAA
  GCGAGTCGCGTCATAGATTCAAGAAAATCTGTCAAGCCAACAATCATTACAGAAGGCGAGGGTAGGGTCA
  TCCTGGGTGAGCCTTCAGCAGTGACCGCTGCATCCGCCGCACAGCGGCGCGGCCGCATTGGAAGGAATCC
  TTCTCAGGTAGGAGATGAGTACTGCTACGGTGGACACACAAATGAGGATGACAGCAATTTTGCTCATTGG
  ACCGAGGCCCGGATCATGCTGGACAATATCAACATGCCTAACGGCCTGATCGCTCAGTTCTATCAGCCGG
  AAAGGGAAAAGGTATATACTATGGATGGCGAATATAGGCTCAGAGGAGAGGAGCGGAAAAACTTTCTGGA
  ACTCTTGCGGACCGCCGACCTGCCTGTTTGGCTTGCTTACAAGGTGGCCGCGGCCGGAGTTTCTTATCAT
  GACAGGAGATGGTGCTTCGACGGCCCACGGACCAACACTATCCTGGAGGACAACAACGAAGTAGAAGTAA
  TAACTAAACTCGGGGAACGGAAAATCTTGCGGCCACGCTGGATCGACGCGAGAGTATACTCCGACCATCA
  GGCGCTCAAAGCCTTCAAGGATTTTGCCAGTGGAAAGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102522 representing YP_001527884
  Red=Cloning sites Green=Tags
  
  MGGVLWDTPSPKEYKKGDTTTGVYRIMTRGLLGSYQAGAGVMVEGVFHTLWHTTKGAALMSGEGRLDPYW
  GSVKEDRLCYGGPWKLQHKWNGQDEVQMIVVEPGKNVKNVQTKPGVFKTPEGEIGAVTLDFPTGTSGSPI
  VDKNGDVIGLYGNGVIMPNGSYISAIVQGERMDEPIPAGFEPEMLRKKQITVLDLHPGAGKTRRILPQII
  KEAINRRLRTAVLAPTRVVAAEMAEALRGLPIRYQTSAVPREHNGNEIVDVMCHATLTHRLMSPHRVPNY
  NLFVMDEAHFTDPASIAARGYISTKVELGEAAAIFMTATPPGTSDPFPESNSPISDLQTEIPDRAWNSGY
  EWITEYTGKTVWFVPSVKMGNEIALCLQRAGKKVVQLNRKSYETEYPKCKNDDWDFVITTDISEMGANFK
  ASRVIDSRKSVKPTIITEGEGRVILGEPSAVTAASAAQRRGRIGRNPSQVGDEYCYGGHTNEDDSNFAHW
  TEARIMLDNINMPNGLIAQFYQPEREKVYTMDGEYRLRGEERKNFLELLRTADLPVWLAYKVAAAGVSYH
  DRRWCFDGPRTNTILEDNNEVEVITKLGERKILRPRWIDARVYSDHQALKAFKDFASGKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009942
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009942.1, YP_001527884
• RefSeq ORF: 1860
• MW: 69.1 kDa