nonstructural protein 2A (NC_009942) Virus Tagged ORF Clone

CAT#: VC102520

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, YP_001527882

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102520 represents NCBI reference of YP_001527882 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATAATGCTGACATGATAGACCCTTTTCAACTGGGCCTGCTGGTAGTATTCCTCGCTACCCAAGAAG
  TGTTGCGGAAGCGCTGGACTGCTAAGATCTCCATGCCTGCTATTTTGATCGCACTGCTGGTTTTGGTGTT
  CGGAGGTATCACCTATACTGACGTGCTGAGATATGTCATTTTGGTAGGCGCGGCTTTCGCCGAGTCAAAT
  AGCGGCGGGGATGTCGTTCATCTCGCTTTGATGGCTACCTTTAAGATTCAGCCCGTCTTCATGGTGGCCT
  CCTTCCTGAAGGCCCGCTGGACTAACCAAGAAAACATCCTGCTTATGCTGGCCGCTGTGTTTTTTCAAAT
  GGCCTACCACGACGCCCGGCAGATCCTTCTGTGGGAAATCCCAGATGTGTTGAACTCCCTTGCTGTCGCT
  TGGATGATCCTGCGCGCCATTACTTTTACCACTACATCTAACGTGGTCGTTCCCCTGTTGGCCCTGCTGA
  CACCCGGACTGAGATGTCTGAACCTCGACGTGTATAGGATCCTGCTCCTGATGGTTGGGATAGGCTCACT
  CATTAGGGAAAAGAGGTCCGCTGCCGCAAAGAAAAAGGGTGCATCACTGCTGTGCCTGGCTCTGGCCAGT
  ACAGGACTGTTTAATCCAATGATTCTCGCAGCTGGTCTGATCGCCTGTGATCCCAATCGGAAAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102520 representing YP_001527882
  Red=Cloning sites Green=Tags
  
  MYNADMIDPFQLGLLVVFLATQEVLRKRWTAKISMPAILIALLVLVFGGITYTDVLRYVILVGAAFAESN
  SGGDVVHLALMATFKIQPVFMVASFLKARWTNQENILLMLAAVFFQMAYHDARQILLWEIPDVLNSLAVA
  WMILRAITFTTTSNVVVPLLALLTPGLRCLNLDVYRILLLMVGIGSLIREKRSAAAKKKGASLLCLALAS
  TGLFNPMILAAGLIACDPNRKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009942
• ORF Size: 696 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009942.1, YP_001527882
• RefSeq ORF: 696
• MW: 25.5 kDa