Human Diazepam Binding Inhibitor (DBI) activation kit by CRISPRa

CAT#: GA101144

DBI CRISPRa kit - CRISPR gene activation of human diazepam binding inhibitor, acyl-CoA binding protein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DBI
• Locus ID: 1622
• Kit Components:

  GA101144G1, Diazepam Binding Inhibitor gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA101144G2, Diazepam Binding Inhibitor gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA101144G3, Diazepam Binding Inhibitor gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001079862, NM_001079863, NM_001178017, NM_001178041, NM_001178042, NM_001178043, NM_001282633, NM_001282634, NM_001282635, NM_001282636, NM_020548, NR_104221, NM_001352432
• Synonyms: ACBD1; ACBP; CCK-RP; EP
• Summary: 'This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'