Human SMARCE1 activation kit by CRISPRa

CAT#: GA104518

SMARCE1 CRISPRa kit - CRISPR gene activation of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SMARCE1
• Locus ID: 6605
• Kit Components:

  GA104518G1, SMARCE1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104518G2, SMARCE1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104518G3, SMARCE1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_003079
• Synonyms: BAF57; CSS5
• Summary: 'The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]'