PRP19 (PRPF19) (NM_014502) Human Mass Spec Standard

CAT#: PH300810

PRPF19 MS Standard C13 and N15-labeled recombinant protein (NP_055317)

Product Images

Coomassie blue staining of purified PRPF19 protein (Cat# TP300810). The protein was produced from HEK293T cells transfected with PRPF19 cDNA clone (Cat# RC200810) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200810
• Predicted MW: 55.2 kDa
• Protein Sequence:
  Sequence Link

  >RC200810 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATS
  IPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGL
  IVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQV
  ASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVF
  SASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSG
  CSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWD
  LRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFI
  ASTGMDRSLKFYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055317
• RefSeq Size: 2342
• RefSeq ORF: 1512
• Synonyms: hPSO4; NMP200; PRP19; PSO4; SNEV; UBOX4
• Locus ID: 27339
• UniProt ID: Q9UMS4
• Cytogenetics: 11q12.2
• Summary: PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]). [supplied by OMIM, Sep 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome, Ubiquitin mediated proteolysis