SOD2 (NM_000636) Human Mass Spec Standard

CAT#: PH302330

SOD2 MS Standard C13 and N15-labeled recombinant protein (NP_000627)

Product Images

Coomassie blue staining of purified SOD2 protein (Cat# TP302330). The protein was produced from HEK293T cells transfected with SOD2 cDNA clone (Cat# RC202330) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202330
• Predicted MW: 24.8 kDa
• Protein Sequence:
  Sequence Link

  >RC202330 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSRAVCGTSRQLAPVLGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQ
  EALAKGDVTAQIALQPALKFNGGGHINHSIFWTNLSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASV
  GVQGSGWGWLGFNKERGHLQIAACPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINW
  ENVTERYMACKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000627
• RefSeq Size: 1593
• RefSeq ORF: 666
• Synonyms: GClnc1; IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6
• Locus ID: 6648
• UniProt ID: P04179, A0A384NL29
• Cytogenetics: 6q25.3
• Summary: 'This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]'
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Huntington's disease