PCYT2 (NM_002861) Human Mass Spec Standard

CAT#: PH303994

PCYT2 MS Standard C13 and N15-labeled recombinant protein (NP_002852)

Product Images

Coomassie blue staining of purified PCYT2 protein (Cat# TP303994). The protein was produced from HEK293T cells transfected with PCYT2 cDNA clone (Cat# RC203994) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203994
• Predicted MW: 43.7 kDa
• Protein Sequence:
  Sequence Link

  >RC203994 representing NM_002861
  Red=Cloning site Green=Tags(s)
  
  MIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDEEIAKHKGPPV
  FTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKR
  TQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQ
  PGETVIYVAGAFDLFHIGHVDFLEKVHRLAERPHIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRY
  VSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRII
  TNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002852
• RefSeq Size: 1856
• RefSeq ORF: 1167
• Synonyms: ET
• Locus ID: 5833
• UniProt ID: Q99447
• Cytogenetics: 17q25.3
• Summary: 'This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]'
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways