PCYT2 (NM_002861) Human Recombinant Protein

CAT#: TP303994

Recombinant protein of human phosphate cytidylyltransferase 2, ethanolamine (PCYT2)

Product Images

Coomassie blue staining of purified PCYT2 protein (Cat# TP303994). The protein was produced from HEK293T cells transfected with PCYT2 cDNA clone (Cat# RC203994) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC203994 representing NM_002861
  Red=Cloning site Green=Tags(s)
  
  MIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDEEIAKHKGPPV
  FTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKR
  TQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQ
  PGETVIYVAGAFDLFHIGHVDFLEKVHRLAERPHIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRY
  VSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRII
  TNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 43.7 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002852
• Locus ID: 5833
• UniProt ID: Q99447
• Cytogenetics: 17q25.3
• Refseq Size: 1856
• Refseq ORF: 1167
• Synonyms: ET; SPG82
• Summary: This gene encodes an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways