EXOSC1 (NM_016046) Human Mass Spec Standard

CAT#: PH306007

EXOSC1 MS Standard C13 and N15-labeled recombinant protein (NP_057130)

Product Images

Coomassie blue staining of purified EXOSC1 protein (Cat# TP306007). The protein was produced from HEK293T cells transfected with EXOSC1 cDNA clone (Cat# RC206007) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206007
• Predicted MW: 21.5 kDa
• Protein Sequence:
  Sequence Link

  >RC206007 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAI
  VTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQS
  NYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057130
• RefSeq Size: 1150
• RefSeq ORF: 585
• Synonyms: CGI-108; CSL4; Csl4p; p13; SKI4; Ski4p
• Locus ID: 51013
• UniProt ID: Q9Y3B2
• Cytogenetics: 10q24.1
• Summary: This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2016]
• Protein Pathways: RNA degradation