ACOT12 (NM_130767) Human Mass Spec Standard

CAT#: PH310445

ACOT12 MS Standard C13 and N15-labeled recombinant protein (NP_570123)

Product Images

Coomassie blue staining of purified ACOT12 protein (Cat# TP310445). The protein was produced from HEK293T cells transfected with ACOT12 cDNA clone (Cat# RC210445) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210445
• Predicted MW: 62 kDa
• Protein Sequence:
  Sequence Link

  >RC210445 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERPAPGEVVMSQAIQPAHATARGELSAGQLLKWIDTTACLAAEKHAGVSCVTASVDDIQFEETARVGQV
  ITIKAKVTRAFSTSMEISIKVMVQDMLTGIEKLVSVAFSTFVAKPVGKEKIHLKPVTLLTEQDHVEHNLA
  AERRKVRLQHEDTFNNLMKESSKFDDLIFDEEEGAVSTRGTSVQSIELVLPPHANHHGNTFGGQIMAWME
  TVATISASRLCWAHPFLKSVDMFKFRGPSTVGDRLVFTAIVNNTFQTCVEVGVRVEAFDCQEWAEGRGRH
  INSAFLIYNAADDKENLITFPRIQPISKDDFRRYRGAIARKRIRLGRKYVISHKEEVPLCIHWDISKQAS
  LSDSNVEALKKLAAKRGWEVTSTVEKIKIYTLEEHDVLSVWVEKHVGSPAHLAYRLLSDFTKRPLWDPHF
  VSCEVIDWVSEDDQLYHITCPILNDDKPKDLVVLVSRRKPLKDGNTYTVAVKSVILPSVPPSPQYIRSEI
  ICAGFLIHAIDSNSCIVSYFNHMSASILPYFAGNLGGWSKSIEETAASCIQFLENPPDDGFVSTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_570123
• RefSeq Size: 1989
• RefSeq ORF: 1665
• Synonyms: Cach; CACH-1; STARD15; THEAL
• Locus ID: 134526
• UniProt ID: Q8WYK0
• Cytogenetics: 5q14.1
• Summary: Hydrolyzes acetyl-CoA to acetate and CoA. [UniProtKB/Swiss-Prot Function]
• Protein Pathways: Pyruvate metabolism