USF1 (NM_207005) Human Mass Spec Standard

CAT#: PH319106

USF1 MS Standard C13 and N15-labeled recombinant protein (NP_996888)

Product Images

Coomassie blue staining of purified USF1 protein (Cat# TP319106). The protein was produced from HEK293T cells transfected with USF1 cDNA clone (Cat# RC219106) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC219106
• Predicted MW: 33.5 kDa
• Protein Sequence:
  Sequence Link

  >RC219106 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQ
  LDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVV
  TTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERR
  RRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQ
  QVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_996888
• RefSeq Size: 1799
• RefSeq ORF: 933
• Synonyms: bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI; UEF
• Locus ID: 7391
• UniProt ID: P22415
• Cytogenetics: 1q23.3
• Summary: 'This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]'
• Protein Families: Druggable Genome, Transcription Factors