AMACR (NM_014324) Human Recombinant Protein

CAT#: TP313437

Recombinant protein of human alpha-methylacyl-CoA racemase (AMACR), transcript variant 1

Product Images

Boxplots of the reactivities of the autoantibodies in ELISA against AMACR (OriGene TP313437) in the sera of prostate cancer cases and healthy controls. Panel B shows the ROC curve for AMACR. Figure cited from Genes Cancer, PMID: 28191285

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  Coomassie blue staining of purified AMACR protein (Cat# TP313437). The protein was produced from HEK293T cells transfected with AMACR cDNA clone (Cat# RC213437) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC213437. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 42.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: ELISA capture for autoantibodies (PMID: 28191285)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_055139
• Locus ID: 23600
• UniProt ID: Q9UHK6
• Cytogenetics: 5p13.2
• Refseq Size: 2534
• Refseq ORF: 1385
• Synonyms: AMACRD; CBAS4; P504S; RACE; RM
• Summary: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis