G CSF (CSF3) (NM_172220) Human Recombinant Protein

CAT#: TP723770

Purified recombinant protein of Human colony stimulating factor 3 (granulocyte) (CSF3), transcript variant 3

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human G-CSF, the region of Thr27-Pro200 from gene Accession# BC033245.1
• Tag: Tag Free
• Predicted MW: 18.6 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.02 - 0.08 ng/ml, corresponding to a specific activity of 1.25 - 5.0 x 10^7 units/mg, determined by M-NFS60 cell proliferation induced by human G-CSF in a dose dependent manner.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_757374
• Locus ID: 1440
• UniProt ID: P09919, Q8N4W3
• Cytogenetics: 17q21.1
• Refseq Size: 1494
• Refseq ORF: 600
• Synonyms: C17orf33; CSF3OS; GCSF
• Summary: 'This gene encodes a member of the IL-6 superfamily of cytokines. The encoded cytokine controls the production, differentiation, and function of granulocytes. Granulocytes are a type of white blood cell that are part of the innate immune response. A modified form of this protein is commonly administered to manage chemotherapy-induced neutropenia. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2020]'
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway