Tcl1 (TCL1A) Human shRNA Plasmid Kit (Locus ID 8115)

CAT#: TL301172

TCL1A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL301172A virus into HEK293 cells. TL301172A virus was prepared using lenti-shRNA TL301172A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL301172B virus into HEK293 cells. TL301172B virus was prepared using lenti-shRNA TL301172B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL301172C] virus into HEK293 cells. [TL301172C] virus was prepared using lenti-shRNA [TL301172C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL301172D] virus into HEK293 cells. [TL301172D] virus was prepared using lenti-shRNA [TL301172D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 8115
• Synonyms: TCL1
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: TCL1A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 8115). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001098725, NM_021966, NR_049726, NM_021966.1, NM_021966.2, NM_001098725.1, BC014024, BC014024.1, BC003574, BC005831, BC009502, BC009891, NM_021966.3, NM_001098725.2
• UniProt ID: P56279
• Summary: Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al., 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al., 2000 [PubMed 10983986]).[supplied by OMIM, Jul 2010]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).