pLenti-EF1a-Cas9-IRES-Puro

CAT#: GE100030

pLenti-EF1a-Cas9-IRES-Puro, Cas9 in lenti vector under EF1a promoter with bicistronic IRES-Puro (10 ug)


Reconstitution Protocol

USD 462.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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CAS9 mouse monoclonal antibody,clone OTI5E5
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Specifications

Product Data
Function Cas9 only vectors
Features

Cas9 only vector with puro selection in lenti backbone

  • 3rd generation Lenti vector, ideal for hard-to-transfect cells
  • Cas9 is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • Lenti particles can be produced using high efficiency lenti packaging kits
  • E. Coli selection: chloramphenicol (34 ug/mL)
Disclaimer These products are manufactured and supplied by OriGene under license from ERS.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.