IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
USD 220.00
2 Weeks
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
USD 340.00
3 Days
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE
| Applications | Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. FLISA: < / = 1.0 µg/ml |
| Reactivities | Rat |
| Conjugation | PE |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP
| Applications | ELISA: 1/4,000-1/10,000. Western blotting. Dot- and Slot-immunoblotting. Immunohistochemistry on Frozen Sections: 1/10-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free
| Applications | ELISA. FLISA: 1 µg/ml. Western blotting. Dot- and Slot-Immunoblotting Immunocytochemistry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
USD 330.00
3 Days
Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Biotin
| Applications | Flow Cytometry: ≤ 1 µg/106 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells. ELISA: 1/5,000-1/20,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
USD 300.00
3 Days
Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, Purified
| Applications | Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells. ELISA. Second step reagent for rat IgG2b Monoclonal Antibodies. |
| Reactivities | Rat |
USD 330.00
3 Days
Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Biotin
| Applications | Flow Cytometry: ≤ 1 µg/106 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells. ELISA: 1/5,000-1/10,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
USD 370.00
3 Days
Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, Purified
| Applications | ELISA. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Frozen Sections. |
| Reactivities | Chicken |
USD 370.00
3 Days
Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified
| Applications | ELISA. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Frozen Sections. |
| Reactivities | Chicken |
Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Purified
| Applications | Immunohistochemistry on Frozen Sections. ELISA: This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent. |
| Reactivities | Rat |
Human IgE mouse monoclonal antibody, clone 4H10, Purified
| Applications | Flow Cytometry: 4 µg/ml. ELISA. |
| Reactivities | Human |
USD 330.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin
| Applications | Flow Cytometry: ≤ 1 µg/106 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. ELISA: 1/5,000-1/20,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
USD 300.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Purified
| Applications | Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. ELISA. Second step reagent for rat IgG1 Monoclonal Antibodies. |
| Reactivities | Rat |
USD 330.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin
| Applications | Flow Cytometry: < / = 0.3 µg/10e6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. ELISA: Can be used as Capture antibody. |
| Reactivities | Rat |
| Conjugation | Biotin |
USD 360.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC
| Applications | FLISA: 1/200-1/400. Flow Cytometry: 1 ≤ µg/106 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. |
| Reactivities | Rat |
| Conjugation | FITC |
USD 300.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified
| Applications | Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. ELISA: Can be used as Capture antibody. Second step reagent for rat IgG1 Monoclonal Antibodies. |
| Reactivities | Rat |
USD 300.00
3 Days
Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Purified
| Applications | Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells. ELISA. Second step reagent for rat IgG2a Monoclonal Antibodies. |
| Reactivities | Rat |
USD 300.00
3 Days
Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified
| Applications | Flow Cytometry. Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells. ELISA. Second step reagent for rat IgG2c Monoclonal Antibodies. |
| Reactivities | Rat |
USD 340.00
3 Days
Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE
| Applications | Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells. ELISA. |
| Reactivities | Rat |
| Conjugation | PE |
Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin
| Applications | Flow Cytometry (< / = 1.0 µg/10e6 cells). Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. ELISA. Second step reagent for rat IgM Monoclonal Antibodies. Surface IgM Detection. B Cell Stimulation. |
| Reactivities | Rat |
| Conjugation | Biotin |
USD 300.00
3 Days
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified
| Applications | Flow Cytometry and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. ELISA. Second step reagent for rat IgM Monoclonal Antibodies. Surface IgM Detection. B Cell Stimulation. |
Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin
| Applications | ELISA: 1/5,000-1/20,000. Flow Cytometry. |
| Reactivities | Rabbit |
| Conjugation | Biotin |
USD 370.00
3 Days
Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified
| Applications | Flow Cytometry: ≤ 1 µg/106 cells. Indirect Immunofluorescence staining. (Ref.1) In ovo suppression of Chicken B lymphocytes. In vitro activation of Chicken peripheral B lymphocytes. |
| Reactivities | Chicken |
USD 450.00
3 Days
Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, Biotin
| Applications | ELISA. Flow Cytometry: < / = 1 µg/10e6 cells. |
| Reactivities | Chicken |
| Conjugation | Biotin |
USD 450.00
3 Days
Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin
| Applications | ELISA. Flow Cytometry: < / = 1 µg/10e6 cells. Immunofluorescence. |
| Conjugation | Biotin |
IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
USD 495.00
5 Days
Chicken IgG / Chicken IgY (heavy chain) mouse monoclonal antibody, clone 16C7, Purified
| Applications | Flow Cytometry. ELISA. Western Blot under non-reducing conditions. |
| Reactivities | Duck |
USD 495.00
5 Days
Chicken IgG / Chicken IgY (light chain) mouse monoclonal antibody, clone 14A3, Purified
| Applications | Flow Cytometry. ELISA. Western Blot. |
| Reactivities | Duck |
USD 475.00
5 Days
Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified
| Applications | ELISA. Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. |
| Reactivities | Human |
USD 450.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Cy5
| Applications | Flow Cytometry: < / = 0.3 µg/10e6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. |
| Reactivities | Rat |
| Conjugation | Cy5 |
USD 360.00
3 Days
Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC
| Applications | Flow Cytometry: < / = 0.3 µg/10e6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells. |
| Reactivities | Rat |
| Conjugation | FITC |
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