Secondary Antibodies

View as table Download

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE

Applications Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
FLISA: < / = 1.0 µg/ml
Reactivities Rat
Conjugation PE

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Human IgE mouse monoclonal antibody, clone BE5, PE

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation PE

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry. 
Immunofluorescence: 
≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation PE

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation PE

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26c, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, PE

Applications Flow Cytometry: < / = 0,2 µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation PE

Rabbit IgG mouse monoclonal antibody, clone 2A9, PE

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Reactivities Rabbit
Conjugation PE

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.1)
Reactivities Chicken
Conjugation PE

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, PE

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 0.1 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgD mouse monoclonal antibody, clone IA6-2, PE

Applications Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities Human
Conjugation PE

Mouse IgG1 goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE

Mouse IgG2a goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE

Mouse IgG2b goat polyclonal antibody, PE

Applications Flow Cytometry: Use 10 µl of 1/20-1/30 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE

Applications Flow cytometry: 0.5 µg/ml.
Reactivities Human
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE