Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP
| Applications | Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis. |
| Reactivities | Chicken |
| Conjugation | HRP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution). Western blot (1/5000). Immunocytochemistry. Immunohistochemistry (1/250). Immunoprecipitation (1/250). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate. |
| Reactivities | Chicken |
| Conjugation | Biotin |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
| Reactivities | Chicken |
| Conjugation | AP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose
| Applications | Immunoprecipitation. |
| Reactivities | Chicken |
| Conjugation | Agarose |
| Immunogen | Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v). |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC
| Applications | Immunocytochemistry (1/200). In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Conjugation | FITC |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
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